A protein with a high percentage of lysine and arginine residues would be best purified and concentrated with which type of column?

Answers

Answer 1

To purify and concentrate a protein with a high percentage of lysine and arginine residues, an ion exchange column would be the best choice. Ion exchange chromatography separates proteins based on their net charge at a specific pH.

In this case, lysine and arginine residues have positive charges at neutral pH. Therefore, an anion exchange column should be used. An anion exchange column is packed with a positively charged resin that binds to negatively charged molecules.
Here's a step-by-step process of purifying and concentrating the protein using an anion exchange column:
1. Prepare the protein sample: Extract and isolate the protein from its source using techniques such as cell lysis and centrifugation.
2. Adjust the pH: Ensure that the pH of the protein sample is compatible with the anion exchange column. Most anion exchange columns work best at neutral or slightly acidic pH.
3. Load the sample: Apply the protein sample to the anion exchange column. The positively charged residues (lysine and arginine) in the protein will bind to the negatively charged resin in the column.
4. Wash the column: Remove any unbound contaminants or impurities by washing the column with a buffer solution. The buffer composition and pH should be optimized to promote binding of the target protein and wash away unwanted substances.
By following these steps, a protein with a high percentage of lysine and arginine residues can be effectively purified and concentrated using an anion exchange column. Remember to consider the specific characteristics of the protein and adjust the conditions accordingly to achieve optimal purification and concentration.

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Related Questions

Will you pls help with this?

Will you pls help with this?

Answers

Answer:

A. Dna thats pased down from parents to offspring

Sonia is riding with her mom in the car and notices an
abandoned baseball field that has a lot of grass and
wildflowers growing in it. What type of succession is she
observing? Explain your answer.

Answers

She is observing an example of secondary succession because the baseball field had some soil and some plant and animal life when it was abandoned.

Which of the following is true about secondary succession?

Secondary succession happens in the opposite direction of primary succession. It begins when processes such as forest fires, hurricanes, and other natural disasters damage the existing ecosystem. Secondary succession is the colonization of a habitat that formerly supported plant and animal life but has since been abandoned owing to ecological disruption. Hurricanes and floods are examples of biological disturbances that can deplete a habitat.

Secondary Succession Examples

Fire. Fire is one of the most prevalent sources of secondary succession and an essential component of the rejuvenation and health of many ecosystems.Crop Land Harvesting, Logging, and Abandonment. Disease Renewal Gap Dynamics.

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Answer:

Sample response: She is observing an example of secondary succession because the baseball field had some soil and some plant and animal life when it was abandoned.

Explanation:) hope this helps have a great day.

brainliest would be appreciated.

How do clouds scatter light energy ?

Answers

Answer:

Non-selective scatter is primarily caused by water droplets in the atmosphere. ... In the visible wavelengths light is scattered evenly, hence fog and clouds appear white. Since clouds scatter all wavelengths of light, this means that clouds block most energy from reaching the Earth's surface.

Explanation:

characteristics of ginseng root

Answers

Answer:

The herb has a light-colored, forked-shaped root, a relatively long stalk, and green leaves in an oval shape. Both American ginseng (Panax quinquefolius, L.)

Explanation:

have a great day!!

The characteristics of ginseng roots include the following:

Fleshy rootsLIght colored forked-shaped rootSturdy tap roots

What is Ginseng?

This is a perennial plant which has a lot of medicinal importance such as

treatment of diabetes etc.

The medicinal properties makes it very valued and it has roots which

have characteristics listed above

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A change in a system that triggers a response that enhances the initial change is:
a. a system in dynamic equilibrium.
b. a negative feedback.
c. a closed system.
d. a positive feedback.

Answers

A change in a system that triggers a response that enhances the initial change is d. a positive feedback.

A positive feedback mechanism is a process in which a change in a system leads to an amplification or enhancement of that change, triggering a response that further promotes or increases the initial change. It results in a self-reinforcing loop where the response reinforces the original stimulus. This can lead to exponential growth or deviation from the initial state.

Unlike negative feedback, which acts to stabilize and restore equilibrium in a system, positive feedback intensifies the original change, driving the system further away from its initial state. Positive feedback loops are commonly observed in biological systems, such as blood clotting, labor contractions during childbirth, and the release of oxytocin during breastfeeding.

While a system in dynamic equilibrium (a) maintains a balance between opposing forces, it does not necessarily involve amplification of the initial change. Negative feedback (b) counteracts changes and restores the system to its original state. A closed system (c) refers to a system isolated from its surroundings, but it does not specify the nature of the feedback mechanism. Therefore, the correct answer is d. a positive feedback.

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over the years the vegetation in africa has grown taller and the giraffe population has also grown taller. Which type of selection is this?

Answers

What type of selection has occurred in this giraffe population?

Directional Selection

Example: Giraffes with the longest necks are able to reach more leaves to each. Selective pressures will work in the advantage of the longer neck giraffes and therefore the distribution of the trait within the population will shift towards the longer neck trait.

Answer:

This is an example of directional selection. Directional selection occurs when individuals at one end of the phenotypic range have higher fitness than individuals in the middle or at the other end of the range. In this case, the increase in vegetation height has provided a selective advantage to giraffes with longer necks, as they can reach leaves high up in the trees. As a result, the average height of the giraffe population has increased over time as individuals with shorter necks are less likely to survive and reproduce. This is an example of directional selection because the trait of interest (neck length) is shifting in one direction (towards longer necks) over time.

Explanation:

How much is DNA important to identify a group? Give a brief explanation on race ,whiteness and property? Does biological anthropologists and genome scientist need to add the relation between Europeans and Indigenous people while doing their research?

Answers

DNA is important in identifying genetic relationships within a group, but it alone is not sufficient to determine complex social constructs like race, whiteness, or property; the inclusion of social, cultural, and historical factors is crucial in understanding these concepts.

Biological anthropologists and genome scientists should consider the relationship between Europeans and Indigenous people in their research to provide a more comprehensive understanding of human genetic diversity and population history.

DNA analysis can provide valuable insights into genetic relationships within a group, such as determining genetic ancestry or identifying related individuals. However, race, whiteness, and property are social constructs that go beyond genetic factors and are shaped by historical, cultural, and socioeconomic factors. These concepts are complex and cannot be solely explained by genetic data. Therefore, it is important for researchers, including biological anthropologists and genome scientists, to recognize the limitations of genetic data and consider the broader social context when studying race, whiteness, and property.

In the context of researching Europeans and Indigenous people, it is crucial for researchers to acknowledge and incorporate the historical and ongoing relationships between these groups. This includes understanding colonization, displacement, and the impact of power dynamics on genetic diversity and health outcomes. By including this relationship in their research, scientists can contribute to a more accurate and nuanced understanding of human genetics and promote social and scientific equity.

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Because of population growth, systems cannot be expanded fast enough to provide sanitation resources for some families. This is an environmental impact on:

A. Air
B. Energy
C. Land
D. Water

Answers

The answer is going to be C
Answer is c pretty sure

Identify the type of plant tissue that brings in gases: epidermal muscle cellulose epithelial

Answers

Gases are brought in and released out through the epidermal tissues

Which of the following best describes a community in the Southern Ocean?
HELP GIVING BRAINLIEST

Which of the following best describes a community in the Southern Ocean?HELP GIVING BRAINLIEST

Answers

Answer:

the antarctic krill, the emperor penguins, and the leopard seals.

Explanation:

The passage says that Antarctic Krill eats up the algae in the underside of sea ice in the Southern Ocean. This means that the Antarctic Krill itself can be found in the Sothern Ocean.

The Emperor penguins and the leopard seals also feed on the Antarctic Krill in the Southern Ocean. This means that both the predators and the prey make up the community in the Southern Ocean.

Minerals are rarely used in everyday life true or false

Answers

Answer: False

Reason: We Use Minerals In our day to day life, when it comes to cooking, or burning. We use Salt to cook amazing meals, and some pots and pans have minerals built in to them so that they last longer over the years you cook with them. When we burn in the fireplace or in the backyard, some of us use coal and light it up, and sometimes, we use minerals to make buildings. In one of my neighboring towns, their City Hall has little mineral pieces built into the City Hall. I hope this helps, and happy holidays! :)

Fill in the blanks:
In water and ice, the slightly ______ hydrogen atoms in one water molecule are drawn to the slightly _______ oxygen atom in another water molecule.

A. positive, negative

B. negative, positive

C. neutral, positive

D. neutral, negative

Answers

Answer:

option c

Explanation:

The water molecule is a polar substance that has partially charged ions. The hydrogen atom is partially positive, whereas the oxygen atom is partially negative in charge. Thus, option A is correct.

What is polarity?

The polarity of the molecule is defined by the presence of the electrical charges over the elemental atom that results in the formation of the chemical bonds by the electrostatic forces.

Water is an example of a polar molecule that is linked by a covalent bond by sharing the electrons between the oxygen and the hydrogen atom of the molecule.

The hydrogen atom is said to have a partial positive charge and oxygen has a partial negative charge because of the uneven electron density. The electronegative oxygen pulls the hydrogen towards it.

Therefore, option A. hydrogen is partially positive and oxygen is partially negative.

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explain the relation between cells, tissues, organs and organ systems in a human body in brief​

Answers

Hi :)

Answer:

The body has levels of organization that build on each other. Cells make up tissues, tissues make up organs, and organs make up organ systems. The function of an organ system depends on the integrated activity of its organs. The survival of the organism depends on the integrated activity of all the organ systems, often coordinated by the endocrine and nervous systems.

Explanation:

In the human body, cells are the basic units of life. Groups of cells working together for a specific function form tissues. Organs are two or more tissues operating together. Even separate organs work together, forming body systems.

Hope this helps :) !!!

Cells form tissues, tissues combine to form organs, and organs work together to create organ systems, collectively maintaining the overall functioning of the human body.

Inside the human body, cells are the fundamental building blocks of life. They're arranged to form tissues. Organs are structures composed of different types of tissue, which perform certain functions. These organs are formed by the combination of tissues.

Organs, in turn, work together to create organ systems. Organ systems are groups of organs that work together in order to perform more extensive functions that allow a body to survive and function properly. Each organ system plays an important role in maintaining homeostasis and interacts with other systems.

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*PLEASE ANSWER!!!*
A non-renewable resource is one that

a.) cannot be replaced in a reasonable amount of time.

b.) creates an increase in average Earth temperatures.

c.) comes from a source that can't be tied to a specific location.

Answers

a.... cannot be replaced in a reasonable amount of time

Answer: a)

A non-renewable resource (also called a finite resource) is a natural resource that cannot be readily replaced by natural means at a quick enough pace to keep up with consumption. 

A folded membrane that forms a network of interconnected compartments in the cytoplasm is called: ______

Answers

A folded membrane that forms a network of interconnected compartments in the cytoplasm is called: Endoplasmic Reticulum(ER)

Endoplasmic Reticulum(ER) is a part of the cell. Every eukaryotic cell contains an Endoplasmic Reticulum. It is a continuous membrane within the cytoplasm of the eukaryotic cell.

Endoplasmic Reticulum can be found in animal cells. In animal cells, more than half of the membranous contents of the cell are usually occupied by Endoplasmic Reticulum. It serves various functions such as helping in a process of synthesis, folding modification, and transport of protein. Depending upon certain physical and functional characteristics Endoplasmic Reticulum can be differentiated into two types, which are called Rough Endoplasmic Reticulum and Smooth Endoplasmic Reticulum.

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how do organs of equilibrium help us maintain our balance? how do organs of equilibrium help us maintain our balance? the macula sends information on head position and the crista ampullaris sends information on rotation movements to the cerebellum for integration. the crista ampullaris sends information on head position and the macula the sends information on rotation movements to the cerebellum for integration. the cerebellum stimulates the organs of equilibrium to help maintain balance. organs of equilibrium stimulate postural muscle to maintain balance.

Answers

Organs of equilibrium stimulate postural muscles to maintain balance. Our organs of equilibrium, which are located in our inner ears, are responsible for helping us maintain our balance and equilibrium. So, the correct answer is C.

The inner ear's macula and crista ampullaris both sense our head's location and any rotational movements. The cerebellum receives this information and analyses it before integrating it.

As a result, the cerebellum signals the equilibrium organs, which in turn activates our postural muscles. Our equilibrium, which keeps us upright and prevents falling, is maintained by these postural muscles.

As a result, our organs of equilibrium are essential in assisting us in keeping our balance.

Complete Question:

How do  organs of equilibrium help us maintain our balance? How do organs of equilibrium help us maintain our balance?

A.. The macula sends information on head position and the crista ampullaris sends information on rotation movements to the cerebellum for integration.

B. The cerebellum stimulates the organs of equilibrium to help maintain balance.

C. Organs of equilibrium stimulate postural muscles to maintain balance.

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Which of the following contributes to sustainability?

A: Driving to work
B: Drinking bottled water
C: Eating more meat
D: Taking a bus to work

Answers

taking a bus to work

The statement that contributes to sustainability is to take a bus to work. Thus, the correct option for this question is D.

What is Sustainability?

Sustainability may be defined as a type of process that significantly fulfills the needs of current generations without compromising the needs of future generations while ensuring a balance between economic growth, environmental care, and social well-being.

Several actions can take that promote the process of sustainability are as follows:

Turn off lights, computers, TVs, radios, and appliances.Lower your thermostat in winter, and raise it in the summer.Set the refrigerator between 38°F and 42°F, and the freezer between O°F and 5°F.Take shorter showers, shut off the water during brushing, and add a brick to your toilet tank.Reduces the dependency on renewable and non-renewable resources.

Therefore, the statement that contributes to sustainability is to take a bus to work. Thus, the correct option for this question is D.

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A leaf from a deciduous forest that falls into a large stream and is consumed by insects would be a source of _______ energy for the stream ecosystem

Answers

A leaf from a deciduous forest that falls into a large stream and is consumed by insects would be a source of primary energy for the stream ecosystem

Flow of energy in the food chain

Most of the energy that enters an ecosystem comes from solar radiation. The producing organisms that carry out photosynthesis absorb solar energy and fix it in chemical energy. When consumers feed on producers, part of that energy goes to these organisms, which, by serving as food for other living beings, also pass on part of their energy to them. This, therefore, flows, in a unidirectional direction, passing from a lower level to a higher one.

With this information, we can conclude that a leaf from a deciduous forest that falls into a large stream and is consumed by insects would be a source of primary energy for the stream ecosystem

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Why a decrease in the maturation of ovarian follicles may lead to reduced implantation rates

Answers

A decrease in the maturation of ovarian follicles may lead to reduced implantation rates because the maturation of ovarian follicles is essential for the release of mature eggs during ovulation. If the follicles do not fully mature, it can result in the release of immature or poor-quality eggs, which may have difficulty fertilizing and implanting in the uterus.

During the menstrual cycle, ovarian follicles develop and mature under the influence of hormones. The maturation process involves the growth and development of the egg within the follicle until it reaches its final stage of maturity. Once the follicle is fully mature, it ruptures, releasing the egg for potential fertilization.

If the maturation of ovarian follicles is compromised or delayed, it can lead to a decrease in the quality and viability of the eggs. This can negatively affect the chances of successful fertilization and implantation in the uterus, ultimately resulting in reduced implantation rates and decreased fertility.

Various factors can contribute to a decrease in the maturation of ovarian follicles, including hormonal imbalances, age-related decline in egg quality, certain medical conditions, or lifestyle factors.

Addressing these underlying causes and seeking appropriate medical interventions may help improve the maturation of ovarian follicles and increase the chances of successful implantation.

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which is the BEST description for the process of photosynthesis?
Photosynthesis is the transfer of matter and flow of energy between the plant and its
environment.
B
Photosynthesis requires an input of matter but not an input of energy
о
С
Photosynthesis requires an input of energy but not an input of matter
D
Photosynthesis is the transfer of matter and flow of energy between the plant and the Sun

Answers

Answer:

Explanation:    

________ is the broadest level of classification and ______ is the most specific.

Answers

Answer:

DOMAIN is the broadest level of classification and ______ is the most specific.

Explanation:

I DONT KNOW THE SECOND ONE SORRY

PLS HELP 20 POINTS

describe how each of the following contributes to structural strength.
a) structural forms
b) structural components
c) structural materials

Answers

The ability of each component, which when combined, forms any structural system, to bear the loads placed on it. the ability of the a structural system could safely transfer a variety of loads to the ground.

How can structural strength be evaluated?

Using a rebound hammer to gauge the concrete's compressive strength is the most typical and ubiquitous method. This bolt is spring-driven and strikes the concrete's surface at a specific speed. Then it more or less vigorously rebounds.

What does Grade 7 science teach about structural strength and stability?

Structural strength refers to a structure's capacity to carry both its own weight and also the weight of any additional stresses. It will crumble or collapse if it doesn't. A structure's capacity to withstand potential external forces is known as structural stability.

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amino acids in a protein polymer are connected by a covalent bond called a [ select ] bond that connect the [ select ] group of the first amino acid to the [ select ] group of the second amino acid. such successive amino acid bondings generate a protein polymer called a [ select ] .

Answers

The amino acids in a protein polymer are connected by a covalent bond called a peptide bond that connects the carboxyl group of the first amino acid to the amino group of the second amino acid. Such successive amino acid bondings generate a protein polymer called a polypeptide.

A peptide bond is formed through a condensation reaction between the carboxyl group of one amino acid and the amino group of another amino acid. This bond results in the formation of a covalent linkage between the carbon and nitrogen atoms of the amino acids, creating a peptide bond. The successive formation of peptide bonds between amino acids leads to the generation of a polypeptide chain, which is the primary structure of a protein.

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PLEASE QUICK!!!!
Complete the analogy

Cells : _______ :: Species : Population

Answers

Answer:

There are more but there is only one blank so,

The answer should be Organism (?)

Which of the following types of mutation, resulting in an error in the mRNA just after the AUG start of translation, is likely to have the most serious effect on the polypeptide product?
a. a deletion of a codon
b. a deletion of 2 nucleotides
c. a substitution of the third nucleotide in an ACC codon
d. a substitution of the fast nucleotide of a GGG codon
e. an insertion of a codon

Answers

The most serious effect on the polypeptide product is likely to occur with option e: an insertion of a codon.

An insertion mutation involves the addition of one or more nucleotides into the DNA sequence, which subsequently affects the mRNA sequence during transcription. In this case, the insertion occurs just after the AUG start codon, which serves as the initiation signal for translation. As a result, the reading frame of the mRNA is shifted, causing a frameshift mutation.

A frameshift mutation alters the grouping of codons, changing the way the genetic code is read during translation. This type of mutation can have drastic consequences because it affects all subsequent codons, leading to a different amino acid sequence in the resulting polypeptide.

In contrast, the other options mentioned, such as a deletion of a codon or substitution of a single nucleotide, can also impact the polypeptide sequence, but they may only affect a specific portion of the protein. The insertion of a codon, on the other hand, disrupts the entire reading frame and has a more significant impact on the final polypeptide product.

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Oxygen on Earth: What is the order for cellular respiration and photosynthesis?

Answers

Answer: While water is broken down to form oxygen during photosynthesis, in cellular respiration oxygen is combined with hydrogen to form water. While photosynthesis requires carbon dioxide and releases oxygen, cellular respiration requires oxygen and releases carbon dioxide.

Explanation:

When you research a topic and write about what you learned without crediting the sources you used it is called what

Answers

Answer:

plagiarism

Explanation:

Answer:

crediting

Explanation:because you gave credit to the place you got it from it is not plagiarism

pls helppp!! Which is the first oxygen-producing organism?

Answers

Answer:

cyanobacteria first evolved

Explanation:

According to certain experts, it is believed that cyanobacteria originated around 2.4 billion years ago.

The first oxygen producing organism is cyanobacteria

c) Suggest what can be done to address any discrepancy. Start explaining the most practical solution first and then the least favorable solution(s). Write in the provided box, please. [10 marks]

Answers

Answer:

Explanation:

yes cux it is good

and i needpoints

what is vegatative propogation?​

Answers

Answer:  Vegetative propagation is an a s exual method of plant reproduction that occurs in its leaves, roots and stem.

Answer:

VEGETATIVE PROPAGATION

regeneration of new plant from the vegetative part or organ of the parent plant is called vegetative propagation vegetative propagation is used full to separate the plant in wider region mother plant get favourable condition if then colonized to the reason by vegetative propagation the offspring adapted

1 NATURAL VEGETATIVE PROPAGATION-

BY ROOT -

the interact root of several Woody plants such as guava sisoo grow just below the soil produce advantages but should just below the soil produce but should develop from this bird which give rise to new plant

BY STEM -

stamp is the source of natural vegetative reproduction in several plants modification or found in stem to perform vegetative propagation

a Runners- the weak stem of creep forward on the soil that downwardly from node axillary bud are present in the axils of scale leaves node

b s ucker - rise time in similar to runner but it is underground its main stem grow upward and develop advantages root from underground

c offset- the thick and fleshy runner of hydrophytes plant is called offset

BY LEAVES

Titan plant develop adventirious bud on their leaves this word after separating from Mother plant give rise to new plant

BY BULBILS

they are fleshy but which are produced in the axils of leaves in the auxiliary Bud

ARTIFICIAL VEGETATIVE PROPAGATION-

GRAFTING

it is the most common method of vegetative reproduction in this method variety of shame spices can be converted into plant of better variety in the technique of trade off to plant are joined in such portion of one part called stock is joint with another plant is called scion

BUDDING-

it is the superior and modern method of plant propagation in this method but of plant are superior varieties grafted in the stem of plant in variety after making oblique you cut up to the bark of stem after fue with this but join with plant as result formed on the new plant

LAYERING-

the method of inductor root formation the branches while they are still attached to a mother plant is called layering

CUTTING -

any part of plant root stem or leaf which has the power to develop itself into a new plant after separating from the mother plant is called cutting

IMPORTANCE OF PROPAGATION

the plant which have lost their power to produce seeds like Banana grapes Rose in it can be propagated by vegetative means only the plant which produce seed in less number are usually propagated vegetative leaf the change of some chemical x-ray and environmental variation as temperature can be studied easily in plant which vegetative propagation the tissue culture technique of vegetative propagation is used to raise disease free plant

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