Agarose gel electrophoresis differs from SDS-PAGE in all of the following ways except loading buffer which generally gives the sample a uniform negative charge. As a result, choice A is the best one.
By determining their size and charge, small molecules (DNA, RNA, protein, etc.) or their fragments can be separated and analysed using the gel electrophoresis technique. Biochemistry as well as molecular biology use it to distinguish between a mixture of fragments of DNA and RNA by length, to determine the size of DNA as well as RNA fragments, or to separate amino acids by charge. Clinical chemistry uses it to separate amino acids by charge and size (IEF agarose, basically size independent). Its loading buffer typically imparts a homogeneous negative charge to the sample.
Therefore, the correct option is option A.
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According to the following reaction, how many grams of ammonia will be formed upon the complete reaction of 29.8 grams of ammonium chloride?ammonium chloride (aq) ammonia (g) + hydrochloric acid (aq)
The grams of ammonia that will be formed upon the complete reaction of 29.8 grams of ammonium chloride is 9.48 grams.
To find the grams of ammonia that will be formed upon the complete reaction of 29.8 grams of ammonium chloride, we need to use the law of conservation of mass and the mole ratio from the balanced chemical equation. The balanced chemical equation for this reaction is:
NH4Cl (aq) → NH3 (g) + HCl (aq)
From the balanced chemical equation, we can see that the mole ratio of ammonium chloride to ammonia is 1:1. This means that for every 1 mole of ammonium chloride that reacts, 1 mole of ammonia will be produced.
Next, we need to find the number of moles of ammonium chloride that are present in 29.8 grams. We can do this by using the molar mass of ammonium chloride:
1 mole NH4Cl = 53.49 g NH4Cl
29.8 g NH4Cl × (1 mole NH4Cl/53.49 g NH4Cl) = 0.557 moles NH4Cl
Now we can use the mole ratio from the balanced chemical equation to find the number of moles of ammonia that will be produced:
0.557 moles NH4Cl × (1 mole NH3/1 mole NH4Cl) = 0.557 moles NH3
Finally, we can use the molar mass of ammonia to find the grams of ammonia that will be produced:
1 mole NH3 = 17.03 g NH3
0.557 moles NH3 × (17.03 g NH3/1 mole NH3) = 9.48 g NH3
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As a result of this process, the proportions of oxygen and carbon dioxide in
air breathed in and air breathed out change.
Which one of the statements is true? Tick the correct box. [1]
- Air breathed out has less carbon dioxide and more oxygen than air breathed in.
- Air breathed out has less carbon dioxide and less oxygen than air breathed in.
- Air breathed out has more carbon dioxide and less oxygen than air breathed in.
- Air breathed out has more carbon dioxide and more oxygen than air breathed in.
Answer:
the third one
Explanation:
When you breathe in, you inhale oxygen and exhale carbon dioxide
what is the volume occupied by 0.897 mol of a gas at 270c (r = 0.08206 l atm/mol k) and 1.45 atm?
The volume occupied by 0.897 mol of a gas at 270°C and 1.45 atm is 21.59 L (rounded to two decimal places).
To find the volume occupied by the gas, we will use the Ideal Gas Law formula: PV = nRT.
In this case, P (pressure) = 1.45 atm, n (moles) = 0.897 mol, R (gas constant) = 0.08206 L atm/mol K, and T (temperature) = 270°C.
First, convert the temperature to Kelvin by adding 273.15: 270 + 273.15 = 543.15 K. Now, plug in the values into the Ideal Gas Law formula:
(1.45 atm) * V = (0.897 mol) * (0.08206 L atm/mol K) * (543.15 K)
To solve for the volume (V), divide both sides by the pressure (1.45 atm):
V = (0.897 mol * 0.08206 L atm/mol K * 543.15 K) / 1.45 atm
V = 21.5867 L
After rounding to two decimal places, the volume occupied by the gas is 21.59 L.
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What is carrying capacity?
Define population.
What environmental problems are
associated with human population
growth?
What events influenced human
population growth?
Answer:
1) Carrying capacity is the maximum number of individuals of a species that an environment can support.
2) Population - all the inhabitants of a particular town, area, or country.
3) An increase in population will inevitably create pressures leading to more deforestation, decreased biodiversity, and spikes in pollution and emissions, which will exacerbate climate change.
4) The three leading causes of population growth are births, deaths, and migration. Births and deaths are seen as natural causes of population change.
HA and HB are two strong monobasic acids. 25.0cm3 of 6.0mol/dm3 HA is mixed with 45.0cm3 of 3.0mol/dm3 HB.
What is the H+ (aq) concentration in mol/dm3 in the resulting solution ?
A 1.9
B 2.1
C 4.1
D 4.5
If anyone could explain how to reach an answer that would be so helpful !!
Answer:
The H+ (aq) concentration of the resulting solution is 4.1 mol/dm³
(Option C)
Explanation:
Given;
concentration of HA, \(C_A\) = 6.0mol/dm³
volume of HA, \(V_A\) = 25.0cm³, = 0.025dm³
Concentration of HB, \(C_B\) = 3.0mol/dm³
volume of HB, \(V_B\) = 45.0cm³ = 0.045dm³
To determine the H+ (aq) concentration in mol/dm³ in the resulting solution, we apply concentration formula;
\(C_iVi = C_fV_f\)
where;
\(C_i\) is initial concentration
\(V_i\) is initial volume
\(C_f\) is final concentration of the solution
\(V_f\) is final volume of the solution
\(C_iV_i = C_fV_f\\\\Based \ on \ this\ question, we \ can \ apply\ the \ formula\ as;\\\\C_A_iV_A_i + C_B_iV_B_i = C_fV_f\\\\C_A_iV_A_i + C_B_iV_B_i = C_f(V_A_i\ +V_B_i)\\\\6*0.025 \ + 3*0.045 = C_f(0.025 + 0.045)\\\\0.285 = C_f(0.07)\\\\C_f = \frac{0.285}{0.07} = 4.07 = 4.1 \ mol/dm^3\)
Therefore, the H+ (aq) concentration of the resulting solution is 4.1 mol/dm³
A substance has an atomic number of 80 and a mass number of 178. How many protons does this atom have? How many neutrons does it have? How did you determine this? Write your answer in ACES format.
Answer:
Cite (source and evidence):
Explain:
Summarize:
Answer:
This atom has 80 protons and 98 neutrons.
Explanation:
All atoms are composed of a central nucleus in which there are positively charged particles, the protons, and neutrally charged particles, the neutrons, around which other negatively charged particles, called electrons, move.
The atomic number, identified with the letter Z, indicates the number of protons that are present in the nucleus of an atom. The atomic number defines the electronic configuration of the atom and allows the ordering of the different chemical elements in the periodic table.
If a substance has an atomic number of 80, this atom has 80 protons.
The mass number indicates the total number of particles in the nucleus, that is, the sum of protons and neutrons.
mass number = number of protons + number of neutrons
In this case you know that the mass number is 178 and number of protons is 80. Then
number of neutrons=mass number - number of protons
number of neutrons= 98
So this atom has 98 neutrons.
A student heated 30 milliliters of water from 0 degrees celcius to 70 degrees celcius. How much energy did she use to heat the water?
The amount of energy used to heat the water from 0°C to 70°C is 8,786.4J.
How to calculate energy?The amount of energy used in heating a substance can be calculated using the following formula:
Q = mc∆T
Where;
Q = amount of heat absorbed or releasedm = mass of substance in gramsc = specific heat of water at 4.184 J/g°C∆T = change in temperatureAccording to this question, a student heated 30 milliliters of water from 0 degrees celsius to 70 degrees celsius.
Since 1mL of pure water equals 1 gram, then 30mL of water will equal 30grams of water.
Q = 30 × 4.184 × (70 - 0)
Q = 8,786.4J
Therefore, 8,786.4J is the energy used to heat the water sample.
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Select the correct answer. Which quantity is a graduated cylinder designed for measuring? A. length B. mass C. temperature D. time E. volume
24. What type of cloud is in the picture?
cumulus
cirrus
altostratus
stratus
Explanation:
alto cumulus is the pic
Calculate the atomic mass of an element if 60.4% of the atoms have a mass of 68.9257 amu and the rest have a mass of 70.9249 amu. Identify the element in the periodic table.
Answer
Explanation
Atomic mass = abundances1 x relative mass 1+ abundances 2 x relative mass 2 ...etc
= ( 60.4% x 68.9257 + 39.6% x 70.9249)
= 0.604 x 68.9257 + 0.396 x 70.9249)
= 69.72 amu
The element with 2 isotopes, one representing 60.4 % of the atoms (68.9257 amu) and the other representing the rest (70.9249 amu), has an atomic mass of 69.7 amu. The element is Gallium.
An element has 2 isotopes. The sum of their abundances is 100%. If the abundance of isotope 1 is 60.4%, the abundance of isotope 2 is:
\(ab_1 + ab_2 = 100 \% \\ab_2 = 100\% - ab_1 = 100 \% - 60.4 \% = 39.6 \%\)
Isotope 1 has an abundance of 60.4% and a mass of 68.9257 amu, while Isotope 2 has an abundance of 39.6% and a mass of 70.9249 amu. The atomic mass is the weighted average of the mass by the abundance.
\(am = \frac{68.9257 amu \times 60.4 + 70.9249 amu \times 39.6 }{100} = 69.7 amu\)
The element with this atomic mass is Gallium.
The element with 2 isotopes, one representing 60.4 % of the atoms (68.9257 amu) and the other representing the rest (70.9249 amu), has an atomic mass of 69.7 amu. The element is Gallium.
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A teacher needs to prepare 4.5 L of 1.0 mol/L ehtanoic acid for an investigation. What volume of concentrated 17.4 mol/L ethanoic acid is required?Select one:a.65 mLb.260 mLc.130 mLd.520 mL
ANSWER
The initial volume of the acid is 260mL
EXPLANATION
Given that;
The final volume of the ethanoic acid is 4.5L
The final concentration of the ethanoic acid is 1.0 mol/L
The initial concentration of the ethanoic acid is 17.4 mol/L
Follow the steps below to find the volume of the ethanoic acid
Step 1; Apply the dilution formula
\(\text{ C1 V1 = C2V2}\)Step 2; Substitute the given data into the formula
\(\begin{gathered} \text{ 17.4 }\times\text{ V1 = 4.5 }\times\text{ 1.0} \\ \text{ 17.4 V1 = 4.5} \\ \text{ Divide both sides by 17.4} \\ \text{ V1 = }\frac{\text{ 4.5}}{\text{ 17.4}} \\ \text{ V1 = 0.260L} \\ \text{ V1 = 260mL} \end{gathered}\)Therefore, the initial volume of the acid is 260mL
adding+specimen+scrapings+to+10%+koh+to+show+the+presence+of+sclerotic+bodies+that+resemble+copper+pennies+is+useful+in+the+diagnosis+of:
Adding specimen scrapings to 10% KOH to show the presence of sclerotic bodies that resemble copper pennies is useful in the diagnosis of tinea versicolor.
Tinea versicolor is a superficial fungal infection caused by the yeast Malassezia. The characteristic coppery or hypopigmented (lighter) spots seen in tinea versicolor are due to the accumulation of fungal cells on the skin. These cells, known as sclerotic bodies or spaghetti and meatballs, are visualized when the specimen scrapings from the affected area are mixed with 10% KOH. The KOH helps to dissolve the keratin in the skin, making it easier to identify the fungal elements under a microscope.
This diagnostic method is particularly effective for tinea versicolor because the presence of these distinctive sclerotic bodies confirms the diagnosis. Other skin conditions may have different appearances or lack these specific fungal elements when examined using the KOH preparation technique. Therefore, this test can aid in differentiating tinea versicolor from other skin conditions with similar clinical manifestations.
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Calculate the maximum wavelength of light capable of dissociating the f–f bond in one molecule of fluorine if the bond energy, or bond dissociation energy, is 157 kj/mol.
The calculated maximum wavelength is 495 nm
The energy needed to break a bond and create two atomic or molecular fragments, each containing one of the original shared pair of electrons, is known as the bond dissociation energy. The link between silicon and fluorine, which was previously discussed, is discovered to be the strongest chemical bond. The initial silicon-fluorine bond in a silicon tetrafluoride molecule requires 166 kcal/mol of bond dissociation energy to be broken.
ΔH=+240 kJ/mol
E=242×103/6.022×1023
=4.0186×10−19 J
Now I use the Planck Expression:
E=hf=hc/λ
∴λ=hc
Eλ=6.63×10−34×3×108/4.0186×10-19 m
λ=4.949×10−7 m
λ=495 nm.
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The electrolyte that is used to make Hypochlorous Acid is made from Sodium Chloride (NaCl). the bottle says that the electrolyte solution contains 25% sodium chloride. If 2.4 ounces of the solution is added to the half gallon to prepare for the reaction. How many total grams of sodium chloride are used in the reaction. If the reaction produces 1100 PPM of HOCl (hypocholorus acid) how many grams of Hypocholrous are made? Do you think all the NaCl was converted to HOCl? or we can brew one more time and get more HOCl. If all of the NaCl converts to HOCl what would be the maximum possible PPM of the disinfectant
Answer:
63.9 grams. Yes, the Nacl was converted. Maximum possible ppm is 540ppm.
Explanation:
I this is college level chemistry not regular high school chem.
Answer:2
Explanation:
it is it do not ask questions
How many atoms of each element are in the following compound:
C3H5(NO3)3
Answer:
Hydrogen: 5
Carbon:3
Nitrogen:3
Oxygen: 9
please give me brainliest
What's the difference between a hypothesis and a theory?
Answer:
C. Theories have been confirmed through tests; hypotheses haven't.
Hope this helps!!
How many moles of glucose did your body consume if you exhale 100 grams of carbon dioxide?
C6H12O6 + 6O2 = 6CO2 + 6H2O
24 g of pure CO2 are exhaled by the individual. To calculate the number of moles, divide the outcome by the molar mass. As a result, the individual exhales about 0.55 moles of carbon dioxide every hour.
What impact on glucose molecule due to exhale of Co2?During aerobic cellular respiration, the interaction of glucose and oxygen results in the production of ATP that the cell can utilize.
As by-products, water and carbon dioxide are produced. The general formula for aerobic cellular respiration is: During cellular respiration, oxygen and glucose combine to create ATP.
CO2 is converted to glucose during photosynthesis, and H2O is oxidized to produce oxygen. Cellular respiration involves the reduction of oxygen and the oxidation of glucose to produce CO2 and water.
CO2 is carried by the bloodstream to the lungs, where it is eventually exhaled out of the body.
Therefore, Hence Number of moles glucose, C6H12O6 = 100g/180g = 0.55 moles.
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g the reagents cl2, alcl3 chlorinate aromatic rings via electrophilic aromatic substitution. considering this reaction, at what position(s) do you expect electrophilic substitution to occur?
The electrophilic substitution of aromatic rings with the reagents Cl2 and AlCl3 typically results in the chlorination of the ring.
The substitution occurs at the ortho and para positions relative to any activating or deactivating groups present on the ring. If the ring is unsubstituted or only has weakly activating groups, then substitution will likely occur at both the ortho and para positions. However, if strongly activating groups are present, substitution may occur exclusively at the para position. The precise location of substitution will depend on the specific properties of the aromatic ring and the reagents used. Electrophilic aromatic substitution with Cl2 and AlCl3 as reagents involves chlorination of aromatic rings. In this reaction, the chlorine (Cl) acts as the electrophile, while AlCl3 serves as the Lewis acid catalyst. The electrophilic substitution typically occurs at the ortho and para positions of the aromatic ring. These positions are more reactive due to the electron-donating nature of substituents already present on the ring, which stabilizes the intermediate formed during the reaction. Overall, electrophilic substitution with Cl2 and AlCl3 targets the ortho and para positions on the aromatic ring.
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1. The electron configuration below represents the ground state of an atom.
2-8-6
This element is found in the Periodic Table in-
Answer:
\( {S}^{16} \)
group 16 period 2 of the periodic table
note: that is not the electronic configuration, that is the Bohr model.
There are various kind of elements that are present in periodic table. Some elements are harmful, some are radioactive, some are noble gases. Sulfur is the correct answer for our given question
What is periodic table?
Periodic table is a table in which we find elements with properties like metals, non metals, metalloids and radioactive element arranges in increasing atomic number.
We can find any element in periodic table if its electronic configuration is given. From electronic configuration, we can find the group number and the period number. So, according to our question the configuration is 2,8,6. Form here we can say that atomic number of element is 16 and the valence electron is 6 so the only group in the periodic table that has valence electron 6 is the group 16 that is oxygen family.
Therefore Element is sulfur and is found in group 16 and period 2
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In lab you have to prepare 530.00 ml solution of 0.125 m copper (ii) oxide. how many grams of solid sodium nitrate must be used to prepare a solution of this concentration?
To prepare a 0.125 M solution of copper (II) oxide with a volume of 530.00 mL, you would need to calculate the mass of solid sodium nitrate required. The molar mass of sodium nitrate (NaNO3) is 85.0 g/mol.
To calculate the mass of solid sodium nitrate needed, you can use the formula:
Mass (g) = Molarity (mol/L) x Volume (L) x Molar Mass (g/mol)
Plugging in the values, we get:
Mass (g) = 0.125 mol/L x 0.530 L x 85.0 g/mol
Simplifying the equation gives:
Mass (g) = 5.31375 g
Therefore, approximately 5.31 grams of solid sodium nitrate must be used to prepare a 0.125 M solution of copper (II) oxide with a volume of 530.00 mL.
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an unknown compound exhibits a signal at 170 ppm in its 13c nmr spectrum. treatment with two equivalents of lialh4 followed by aqueous workup gives an amine. what could be the identity of the unknown compound?
The existence of a carbonyl group in the unidentified chemical is indicated by the signal at 170 ppm in the 13C NMR spectra.
What number of peaks or signals would you anticipate to see in the ethyl acetate C-13 NMR spectrum?It displays the four anticipated signals one for each of the carbonsas expected.
How many peaks does a carbon atom in a chemical cause the 13C NMR spectrum to produce?Four carbons and four peaks are present. No two carbons exist in the same environment exactly. The peak at slightly over 50 must be a carbon with a single bond connecting it to an oxygen.
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Explain, in terms of particles and collisions, the effect that increasing the surface area of the marble chips has on the rate of reaction.
I will mark brainest need asap
Answer:
increase rate of reaction
Explanation:
because marble chips has been broken into small pieces
Automobile airbags contain solid sodium azide, nan3, that reacts to produce nitrogen gas when heated, thus inflating the bag.
The values of work, w, for the system if 16.5 g NaN 3 reacts completely at 1.00 atm and 22 ∘ C. The work done is -919 J.
The equation of the reaction is;
2 NaN 3 ( s ) ⟶ 2 Na ( s ) + 3 N 2 ( g )
Number of moles of NaN3 = 16.5 g/65 g/mol = 0.25 moles
If 2 moles of NaNa3 yields 3 moles of N2
0.25 moles of NaN3 yields 0.25 moles × 3 moles/2 moles
= 0.375 moles of N2
We need to find the volume change using the;
PV = nRT
P = 1.00 atm
V =?
n = 0.375 moles of N2
R = 0.082 atmLK-1mol-1
T = 22 ∘ C + 273 = 295 K
V = nRT/P
V = 0.375 × 0.082 × 295/ 1.00
V = 9.07 L
Recall that during expansion the gas does work. Work done by the gas is;
W = -PΔV
W =-( 1 atm × 9.07 L)
W = -9 Atm
Again;
1 L atm = 101.325 J
So,
-9 atmL = -9 atmL × 101.325 J/1 L atm
= -919 J
The work done is -919 J.
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Disclaimer:- your question is incomplete, please see below for the complete question.
Automobile airbags contain solid sodium azide, NaN 3, that reacts to produce nitrogen gas when heated, thus inflating the bag. 2 NaN 3 ( s ) ⟶ 2 Na ( s ) + 3 N 2 ( g ) Calculate the value of work, w, for the system if 16.5 g NaN 3 reacts completely at 1.00 atm and 22 ∘ C.
2) A sample of argon has a volume of 5.0 Land the pressure is 650 mm Hg If the final temperature is
30. C, the final volume is 5.7 L, and the final pressure is 800. mm Hg, what was the initial temperature
of the argon?
Answer:
\(T_1 = -57.15^{\circ}C\)
Explanation:
Given
\(P_1 = 650mmHg\) --- Initial Pressure
\(V_1 = 5.0L\) --- Initial Volume
\(V_2 = 5.7L\) --- Final Volume
\(P_2 = 800mmHg\) --- Final Pressure
\(T_2 = 30C\) ---- Final Temperature
Required
Determine the initial temperature (T1)
This question will be solved using combined gas law which states:
\(\frac{P_1V_1}{T_1} = \frac{P_2V_2}{T_2}\)
However, the final temperature must be converted to degree kelvin
\(T_2 = 30C\) --- Add 273.15
\(T_2 = 30k + 273.15 k\)
\(T_2 = 303.15k\)
Make T1 the subject in \(\frac{P_1V_1}{T_1} = \frac{P_2V_2}{T_2}\)
\(T_1 = \frac{P_1V_1T_2}{P_2V_2}\)
Substitute values for P1, V1, T2, P2 and V2
\(T_1 = \frac{650 * 5.0 * 303.15}{800 * 5.7}\)
\(T_1 = \frac{985237.5}{4560}\)
\(T_1 = 216.060855263\)
Approximate
\(T_1 = 216k\)
Convert to degree Celsius
\(T_1 = 216k\) --- Subtract 273.15
\(T_1 = 216 - 273.15\)
\(T_1 = -57.15^{\circ}C\)
Hence, the initial temperature is -57.15C
ide appreciate if someone would help me with these.
Answer:
can't do much
Explanation:
baking soda;8,alkaline
bleach:12,alkaline.
coffee:5.10,acidic
coke;coke,acidic
milk;6.9,acidic.
lemon juice;2,acidic
this is all i can do.
A scientist is measuring the pressure that is exerted by each of the following gases in the atmosphere: carbon
dioxide, oxygen, and nitrogen. Which term most likely describes what she is measuring?
-final pressure
-atmospheric pressure
-combined pressure
-partial pressure
The term that describes what she is measuring is called partial pressure.
What is the Dalton law of partial pressure ?From the Dalton's law of partial pressure, the total pressure of a gas is regarded as the sum of the partial pressures of all the gases in the mixture.
As such, each of the gases; carbon dioxide, oxygen, and nitrogen all possess partial pressures thus the term that describes what she is measuring is called partial pressure.
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Which statement about Niels Bohr's atomic model is true?
Correct answer is Each orbit has a specific energy level.
According to Bohr's atomic model There is a certain energy for each orbit, with the inner orbit having the lowest energy. As you move farther from the nucleus, the energy of the orbits increases. We can say that the energy of the electrons are quantized if we state that they can only have certain energies.The ground state, also known as the lowest orbit, is where the electron typically resides.A lower energy level to a higher energy level is reached by an atom's electrons by gaining the necessary energy, and a higher energy level to a lower energy level by losing energy.Inner energy levels have low energy and outer or higher energy levels have high energy.Therefore, Correct statement is Each orbit has a specific energy level.
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The complete question is mentioned below:
Which statement about Niels Bohr’s atomic model is true?
a) Higher orbits have lower energies.
b) Each orbit has a specific energy level. c) Electrons can exist in any energy level. d) Orbits close to the nucleus have no energy.
Which statement best describes the relationship between kinetic energy, mechanical energy, and potential energy?
A.
Kinetic energy is part of mechanical energy, but potential energy is not.
B.
Kinetic energy is part of potential energy, but mechanical energy is not.
C.
Kinetic energy and potential energy are the two kinds of mechanical energy.
D.
Kinetic energy and mechanical energy are the two kinds of potential energy.
Answer:
C
Explanation:
because kinetic and potential are both parts of mechanical energy
you should know we learned it in class
Photosynthesis is the chemical process in which plants and other organisms
combine carbon dioxide and water, using energy from the Sun, to produce
___and___
1.sugar
2.oxygen
3.sugar and oxygen
Answer:
sugar and oxygen
Explanation:
sugar (glucose) and oxygen
One mole of react and reacts with the rate of 0. 6 mole per dm cube per second what is the rate constant of this reaction if reaction is first order 1 per second 0. 3 per second 0. 6 per second 0. 9 per second
Bond length and Bond distance is the equilibrium distance between the two atoms which forms a covalent bond. The correct answer is 0. 6 per second.
It is typically given in mm way NM bond strength of bond dissociation strength is the strength wished to interrupt a covalent bond among atoms of a diatomic covalent compound in its gaseous state. A first-order response may be described as a chemical response wherein the response charge is linearly depending on the awareness of simplest one reactant. In different words, a first-order response is a chemical response wherein the charge varies primarily based totally at the adjustments withinside the awareness of simplest one of the reactants.
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