Describe the process of how the PTC receptor gene is replicated and verified using PCR technology and an agarose gel. In detail, explain the general materials (at least 5 materials other than lab equipment or containers) needed to conduct a PCR reaction giten DNA that you extracted from cheek cells.
Answers
The process of replicating and verifying the PTC receptor gene using PCR technology and agarose gel involves several steps. DNA is extracted from cheek cells, and PCR is used to amplify the specific region of the PTC receptor gene. The PCR products are then analyzed using agarose gel electrophoresis to verify the presence and size of the amplified DNA fragments. The necessary materials for the PCR reaction include primers, nucleotides, DNA polymerase, PCR buffer, and template DNA.
To begin the process, DNA is extracted from cheek cells using a DNA extraction kit or a method like the phenol-chloroform extraction. Once the DNA is isolated, a PCR reaction is set up to amplify the specific region of the PTC receptor gene. The materials required for the PCR reaction include:
Primers: Two specific DNA primers are designed to flank the target region of the PTC receptor gene. These primers are complementary to the DNA sequence and serve as starting points for DNA amplification.
Nucleotides: The four DNA building blocks (A, T, C, and G) called nucleotides are required for DNA synthesis during PCR. They are added in excess to ensure efficient amplification.
DNA polymerase: A thermostable DNA polymerase, such as Taq polymerase, is used in PCR to catalyze DNA synthesis. This enzyme can withstand the high temperatures required for denaturation and extension during each PCR cycle.
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Related Questions
1 a)which association regarding the function and location of the cerebrum is most accurate?
a)sensory-posterior
b)motor-medial
c)motor-posterior
d)sensory medial
1b)the term peripheral nervous system (PNS) refers to the
a)brain and spinal cord
b)spinal nerves,cranial nerves, and peripheral ganglia
c)brain spinal cord and periphereal nerves
d)spinal cord ans spinal nerves
Answers
1a) Motor-medial association regarding the function and location of the cerebrum is most accurate. The correct option is (b).
1b) b) the term peripheral nervous system (PNS) refers to the spinal nerves, cranial nerves, and peripheral ganglia. The correct option is (b).
Explanation for 1a:
The cerebrum is the largest part of the brain, and it is divided into two hemispheres, each of which is divided into four lobes. The motor cortex, which controls voluntary movements, is located in the frontal lobe of the cerebrum, specifically in the medial region.
Therefore, the association between motor function and the medial region of the cerebrum is most accurate.
Explanation for 1b:
The peripheral nervous system (PNS) refers to the parts of the nervous system that are outside of the brain and spinal cord. It includes the 12 pairs of cranial nerves, which originate from the brain, and the 31 pairs of spinal nerves, which originate from the spinal cord.
The PNS also includes the peripheral ganglia, which are groups of nerve cells located outside of the brain and spinal cord. The PNS is responsible for carrying sensory and motor information between the body and the central nervous system.
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Which method is most likely used to identify what happened before the start of the Paleozoic era? Scientists study fossils from the Jurassic period. Scientists observe index fossil records of the Mesozoic era. Scientists examine fossil evidence during the Precambrian time. Scientists explore the emergence of mammals and humans in the Cenozoic era.
Answers
Scientists examining the fossil evidence during the Precambrian time is used to identify what happened before the start of the Paleozoic era.
What is Precambrian time?This is defined as the period of time which extends from about 4.6 billion years ago.
Due to the wide difference in time present, its fossil is used to identify what happened before the start of the Paleozoic era which is why option C was chosen.
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Scientists examine fossil evidence during the Precambrian time, is used to know observation before the start of the Paleozoic era, hence option C is correct.
What is fossil evidence during the Precambrian time?Prior to 541 million years ago, the Precambrian was known as the "Age of Early Life." The oceans of the planet were home to soft-bodied animals like worms and jellyfish.
The land remained uncultivated. Microorganisms, primitive creatures, and stromatolites, mats of algae, are typical Precambrian fossils.
They may be able to determine what occurred before the Paleozoic era by doing research on events during the Precambrian period.
Therefore, scientists examine fossil evidence during the Precambrian time, hence option C is correct.
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mature nerve cells, which are incapable of cell division, are most likely in
Answers
Mature nerve cells, which are incapable of cell division, are most likely in the G0 phase of the cell cycle.
Mature nerve cells are cells that have developed to the point of being ready for use. They are cells that have differentiated, meaning that they have taken on the characteristics of a particular cell type, in this case, nerve cells. As a result of the cell division cycle, these mature nerve cells have ceased dividing and are incapable of cell division. They are in a quiescent state, referred to as the G0 stage. It is the stage in the cell cycle where cells are no longer dividing, yet they remain metabolically active and functional.
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15. Each of the following is a density-dependent limiting factor EXCEPT
A. crowding
B. competition
C. disease
D. seasonal cycles
Answers
Answer: d, seasonal cycles
Explanation: it doesn't affect the population.
What is the water cycle? (2 points) a A process by which water moves from cloud to cloud in the sky b A process by which water is absorbed into soil on Earth's surface c A process by which water moves from Earth's surface into the sky and back again d A process by which wind washes large amounts of water onto land from the oceans (HELP PLEASEEEE)
Answers
Answer: The correct answer is C...I made a typo error in my original so i want to make clear that it is C. A process by which water moves from Earth's surface into the sky and back again
Explanation:
The water cycle is a process involving precipitation, evaporation, and condensation.
This is the concept in a nutshell:
Precipitation is rain/snow/sleet, but mostly classified as rain. It means when the water in the clouds rains down and hits the ground. Evaporation is the process of the water that hits the ground invisibly going back up into the sky and drying from the ground. From this point, the evaporated water condenses into clouds, hence the name condensation. From this point forward, the water precipitates again, evaporates again, condense again, and repeat
what is the scientific name of seri silkworm?
Answers
Answer:
Bombyx mori
Explanation:
Answer:
Bombyx mori
Explanation:
complete the following paragraph to describe the impact of mutations on the hemoglobin protein. answer choices may be used more than once or not at all.
Answers
Mutations in the hemoglobin protein can lead to structural changes, altered oxygen affinity, and the development of blood disorders.
Mutations in the hemoglobin protein can have significant impacts on its structure and function. One example of a mutation that affects hemoglobin is the sickle cell mutation, where a single nucleotide change leads to the substitution of glutamic acid with valine in the hemoglobin molecule.
This mutation alters the shape of the hemoglobin protein, causing it to form long, rigid fibers under certain conditions. These fibers distort the shape of red blood cells, giving them a characteristic sickle shape. The altered structure of sickle hemoglobin affects its oxygen-carrying capacity, leading to reduced oxygen delivery to tissues and organs.
Overall, mutations in the hemoglobin protein can impact its structure, oxygen affinity, and overall function. These changes can contribute to the development of blood disorders, such as sickle cell disease and thalassemias, which can have significant health implications for affected individuals.
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Scientists use results from new experiments to
support their own personal political opinions.
increase precision of data that were collected previously.
modify existing explanations of how nature works.
aid in distinguishing science from pseudoscience.
Answers
Answer:
B
Explanation:
Answer:
C
Explanation:
Edge 2021
A medical student finds similarities between a current conflict with another student and past conflicts in order to assist in a resolution. What else does a critical thinker do to resolve conflict? Check all that apply.
observes the situation
evaluates information
argues one’s point
Answers
Answer:
c
Explanation:
Initial temperature (°C) Final temperature (°C)
21.6
15.6
21.6
15.6
Which statement is correct?
Thermal energy was transferred more slowly out of the uncovered aquarium,
Thermal energy was transferred more slowly out of the covered aquarium,
Or thermal energy was transferred out of both aquariums at the same rate.
Answers
Thermal energy was transferred more slowly out of the uncovered aquarium statement is correct
What happens if a liquid receives extra heat energy?Atoms or molecules in a liquid move more quickly and apart from one another when thermal energy is provided. The density of molecules can be reduced and they can leave the liquid state to become gas molecules thanks to the rise in thermal energy. When a material transforms from a gas to a liquid, condensation takes place.
Convection is a method of heat transmission that moves heat energy away from the source of heat by causing a fluid, such as a gas or liquid, to move. A substance's particles vibrate more when it is heated.
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Data about plant and animal cell and diffrences
Answers
Hi.
Plant cell has chloroplast.
Animal cell do not.
Plant cell has a large vacuole.
Animal cell has a small vacuole.
Plant cell has a cell wall.
Animal cell do not.
Hope it helps. Brainliest please.
please helppppp if greenhouse gas concnetration is low, will temperature be low?
Answers
Answer:
yes me boi
Explanation:
SOMEONE WANNA TELL ME IF MY PROJECTS GOOD?
Answers
What I think:
I think your answers are great!
Why I think this:
I tried figuring it out and I kept getting the same answers that you got!
My notes:
I hope this helped you! Also, great job! Can I please be marked as Brainliest? Thanks!
What are possible causes of cystic fibrosis?
AR
Select all that apply.
too many CFTR proteins being produced by cells
a faulty CFTR gene
the existing CFTR proteins being too aggressive at their job
CFTR proteins not being produced or functioning properly
Answers
These actions can inhibit the effectiveness on the cell membrane.
Some bacteria cells divide at a rate of
Answers
Answer:
Each daughter cell is a clone of the parent cell. When conditions are favorable such as the right temperature and nutrients are available, some bacteria like Escherichia coli can divide every 20 minutes. This means that in just seven hours one bacterium can generate 2,097,152 bacteria.
how does the mitochondria lysosomes and golgi apparatus work together
Answers
Answer:
The mitochondria segregate the Golgi from lateral regions of the plasma membrane, the nucleus, and the cytoplasm. The mitochondria and the golgi structures from very close contacts, and these contacts remain stable over time.
Which of the following set is the correct combination of organs that do
not carry out any digestive functions? (1)
A) Oesophagus, Large Intestine, Rectum
B) Buccal Cavity, Oesophagus, Rectum
C) Buccal Cavity, Oesophagus, Large Intestine
D) Small Intestine, Large Intestine, Rectum
Answers
The Answer Is B
buccal Cavity , Oesophagus , Rectum
A fish farmer has a large pool used to grow a species of fish. The farmer decides to add a second species of fish to the pool. Both fish species feed on the same type of food, but the fish farmer does not increase the amount of food added to the pool, maintaining the same carrying capacity in the pool.
Which graph shows how the population of the two fish species will change?
Answers
Answer:
D
Explanation:
Explanation: The capacity of fish to the ratio of population change increased and showed a variable degree of measurements.
he adaptive immune response is divided into two branches. The first is – immunity, in which antibodies are produced that bind to – associated with the foreign cell. In the second branch, – immunity, – recognize foreign cell structures and may either destroy infected – cells or help activate –, which can then generate plasma cells that produce
Answers
Answer:
The adaptive immune response is divided into two branches. The first is immunity, in which antibodies are produced that bind to the foreign cell. In the second branch, immunity, recognizes foreign cell structures and can destroy or help activate infected cells, which can generate plasma cells that produce specific antibodies.
Explanation:
Specific antibodies are those proteins that mark certain pathogens, these are manufactured by cells of the immune lineage, and the acquired defense, that is, the one acquired by humans during their growth, is not born with these antibodies.
Specific antibodies are formed once a pathogen has been recognized, that is, in the second encounter between the pathogen and the organism, that is why we are talking about a first infection and a second infection.
These antibodies are recognized as those that are formed in some vaccines.
compensation mechanisms in the body for dehydration would include: group of answer choices peripheral vasodilation. decreased aldosterone. x increased antidiuretic hormone (adh). slow, strong heart contraction.
Answers
The compensation mechanisms in the body for dehydration include increased antidiuretic hormone (ADH) secretion. Option C is correct.
Antidiuretic hormone (ADH) secretion stimulates water reabsorption by the kidneys, leading to reduced urine output and increased blood volume. This response helps to maintain blood pressure and prevent further fluid loss.
Peripheral vasodilation is not a compensatory mechanism for dehydration, as it would further decrease blood volume and blood pressure. Instead, vasoconstriction may occur to help maintain blood pressure.
Decreased aldosterone secretion is not a typical compensatory mechanism for dehydration, as aldosterone stimulates sodium and water reabsorption in the kidneys, which would be helpful in maintaining blood volume.
A slow, strong heart contraction may occur in response to dehydration in an attempt to compensate for decreased blood volume and maintain blood pressure, but it is not a direct compensation mechanism for dehydration.
Hence, C. is the correct option.
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--The given question is incomplete, the complete question is
"Compensation mechanisms in the body for dehydration would include: group of answer choices A) peripheral vasodilation. B) decreased aldosterone. C) increased antidiuretic hormone (ADH). D) slow, strong heart contraction."--
Hydro power is another source of renewable energy. It relies on two forms of energy involved in falling or flowing water. Identify the two forms of energy that hydro power relies on. Explain your response.
Answers
Answer:
Potential and kinetic energy
Explanation:
Hydropower use to potential energy and kinetic energy to produce electricity. It uses the potential energy of the water store in dam and this potential energy is the energy posses by an object at rest. The potential energy is now converted to kinetic energy when the water begin to flow through it pipes and began to turn the turbines which then convert it to mechanical rotation of the turbine.
The epithelial cells of the small intestine have large numbers of mitochondria.
Explain how this is an adaptation for the function of these cells.
Answers
Answer:
★ - Mitochondria is site of ATP production.
★ - High respiration rate if there are lots of them for active transport.
★ - Needed for the metabolic activity - absorption of digested foods
Explanation:
Hope you have a great day ;-)
Mitochondria is the power house of ATP production, The epithelial cells of the small intestine have large numbers of mitochondria as it is needed for the metabolic activity like absorption of digested foods.
What are the function of mitochondria ?Mitochondria is the power house of the cell which is made up of double membranes responsible for production of energy, contain proteins, lipids, and nucleic acids.
If mitochondria is destroyed, the cell dies and the outer membrane of mitochondria is very similar to the nuclear membranes the nucleus where the inner membrane is bigger and forms multiple folds called cristae.
The area present between two membranes is called the matrix, have circular DNA, multiple proteins and essential molecules, it take of sugars by cellular proteins, enter Krebs cycle produces smaller molecules.
When electron moved a spike of energy is generated called as ATP.
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PLEASE ANSWER ASAP!!!!!!!!!!!!!!!!The video shows the movement of oil near the site of the 2010 oil spill. Oil near an oil spill This video shows the shoreline a distance away from this spill. Shoreline away from an oil spill What happens to the oil after it is spilled?
Answers
When oil is spilled, it can have a devastating impact on the environment. The oil spreads over a wide area, coating plants, animals, and shorelines and also enter the food chain, harming both marine life and humans.
What can be damaged from oil spill?There are a number of things that can happen to oil after it is spilled. Some of the oil will evaporate, but most of it will remain in the environment. The oil that remains can be broken down by bacteria, but this process can take many years.
In the meantime, the oil can cause a number of problems. It can smother plants and animals, making it difficult for them to breathe and survive. It can also contaminate water supplies, making them unsafe to drink or use for bathing.
The 2010 oil spill in the Gulf of Mexico is a good example of the damage that oil spills can cause. The spill released over 200 million gallons of oil into the Gulf, and it took years to clean up the mess. The spill had a devastating impact on the environment, killing thousands of birds, fish, and other marine animals. It also caused economic damage to the Gulf Coast region, as tourism and fishing were disrupted.
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In 3-5 sentences describe the relationship between a glucose molecule and the products it makes during cellular respiration
Answers
During Cellular Respiration, glucose is broken down into many different pieces turning into pyruvate and lead to release of electrons. During cellular respiration, this is a process known as glycolysis which takes place in cytoplasm. The pyruvate then oxidizes (meaning it loses the electrons which produce energy) and turns into acetyl CoA which is used in Krebs/citric acid cycle.
Cellular respiraton is a chain of chemical reactions that smash down glucose to produce ATP, which may be used as strength to strength many reactions throughout the frame. There are 3 foremost steps of cellular breathing: glycolysis, the citric acid cycle, and oxidative phosphorylation.
Cellular respiration is the process that happens inside the mitochondria of organisms (animals and flora) to interrupt down sugar inside the presence of oxygen to release electricity inside the form of ATP. This technique releases carbon dioxide and water as waste products.
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What are the types of reproduction? What are the effects of Gonorrhoea on the female reproductive system?
Answers
Answer:
1.) Asexual and sexual
Untreated gonorrhea can lead to Infertility in women and can cause pelvic inflammatory disease. Pelvic Inflammatory Disease (PID) can result in scarring of the tubes, greater risk of pregnancy complications and infertility.
what is the term used for populations moving into an area
Answers
Answer:
Popularity Growth (Immigration)
Explanation:
:( i tried (sorry)
Answer
Population transfer or resettlement is the movement of a large group people from one region to another
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Which of the following human impacts could cause Eutrophication of water bodies? Deforestation farming burning fossil fuels or all of the above
Answers
A process known as eutrophication is brought on by an excess of nutrients, primarily nitrogen and phosphorus, in water. Algae thrive, spread, and color the water green as a result of feeding on the nutrients.
Which 3 human factors contribute to eutrophication?Because of human activities, cultural eutrophication is a process that accelerates natural eutrophication. The three primary sources for anthropogenic nutrient input are sewage form cities and industrial waste water, as well as erosion and leakage from fertilized agricultural areas.
Use of fossil fuels in deforestationMore carbon dioxide is now accumulating in the atmosphere as a result of burning fossil fuels than can be absorbed by current carbon sinks like forests, as well as the loss of carbon sinks from deforestation and other activities.
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African fish eagles are large raptors that have a wide range across sub-Saharan Africa. Which of these organisms do you think would have a niche similar to that of an African fish eagle?
A- American bald eagle
B- European blue jay
C- White-tailed deer
D- Mountain lion
Answers
The organism that would have a niche similar to that of an African fish eagle is the American bald eagle (option A).
What is a niche?A niche refers to a function within an ecological system to which an organism is especially suited.
The niche of an organism distinguishes that organism from other organisms and ensures its adaptation and survival.
According to this question, African fish eagles are large raptors that have a wide range across sub-Saharan Africa. They consume fishes mostly as preys.
This suggests that the organism that would have a niche similar to that of an African fish eagle is another organism with similar characteristics such as the American bald eagle.
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What i a hypothei for A bagged chemical reaction where you will be mixing tuff in a bag?
Answers
If the reactants in the chemical reactions are known, then the products can be predicted because the pattern for chemical reactions is followed.
Calcium carbonate, sodium chloride, and carbon dioxide gas are the products of the chemical reaction. This alters the pH inside the bag, which causes the phenol red's color to change. To demonstrate the generation of the gas, as much air as feasible should be taken out of the bag.A straightforward hypothesis just hypothesizes the link between two independent and dependent variables. Examples: The next day you feel exhausted if you stay up late. Your phone will charge more quickly if you turn it off.To know more about chemical reaction here
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NEED THIS ASAP!!!! Define: Meiosis, Sister chromosomes, homologous chromosomes
Answers
Answer:
meiosis- a type of cell division that results in four daughter cells each with half the number of chromosomes of the parent cell, as in the production of gametes and plant spores.
sister chromosomes- A sister chromatid refers to the identical copies (chromatids) formed by the DNA replication of a chromosome, with both copies joined together by a common centromere.
homologous chromosomes- Homologous chromosomes are two pieces of DNA within a diploid organism which carry the same genes, one from each parental source. Each parent provides the same 23 chromosomes, which encode the same genes.
Answer:
Meiosis is a process where a single cell divides twice to produce four cells containing half the original amount of genetic information. These cells are our sex cells – sperm in males, eggs in females. ... These four daughter cells only have half the number of chromosome
A sister chromatid refers to the identical copies formed by the DNA replication of a chromosome, with both copies joined together by a common centromere. In other words, a sister chromatid may also be said to be 'one-half' of the duplicated chromosome. A pair of sister chromatids is called a dyad
homology is similarity due to shared ancestry between a pair of structures or genes in different taxa.
Chromosomes are the things that make organisms what they are. They carry all of the information used to help a cell grow, thrive, and reproduce. Chromosomes are made up of DNA. Segments of DNA in specific patterns are called genes. You will find the chromosomes and genetic material in the nucleus of a cell.
Explanation:
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