Will the ampR promoter drive the ampC expression as a selection
marker to allow for resistance to ampicillin? Is this how ampR
promoter plays a role in engineered plasmid applications

Answers

Answer 1

The ampR promoter drives the ampC expression as a selection marker to allow for resistance to ampicillin, and this is how the ampR promoter plays a role in engineered plasmid applications.

"Will the ampR promoter drive the ampC expression as a selection marker to allow for resistance to ampicillin? Is this how ampR promoter plays a role in engineered plasmid applications the ampR promoter drives the ampC expression as a selection marker to allow for resistance to ampicillin. This is how the ampR promoter plays a role in engineered plasmid applications.

The ampR promoter drives the ampC expression as a selection marker to allow for resistance to ampicillin. This is because the gene that is responsible for beta-lactamase production (ampC) is regulated by the ampR gene.The ampR gene regulates ampC expression. If ampicillin is present in the environment, the beta-lactamase will degrade it, which will result in resistance to the antibiotic.AmpR is also used as a selection marker in plasmid engineering applications. This is because, when used in combination with an antibiotic resistance gene, the transformed bacterial cells will become resistant to the antibiotic that was used during the selection process.

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Related Questions

which choice is not a mechanism of gene regulation that can take place after transcription is completed in eukaryotes?

Answers

A Pre-mRNA isn't always a mechanism of gene regulation that may take place after transcription is finished in eukaryotes.

Gene expression in eukaryotic cells is regulated via repressors as well as by transcriptional activators. Like their prokaryotic opposite numbers, eukaryotic repressors bind to unique DNA sequences and inhibit transcription.

In particular, gene expression is managed in two ranges. First, transcription is controlled by proscribing the amount of mRNA that is comprised of a selected gene.

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(Physical science! Help!)

(Physical science! Help!)

Answers

Answer:

b

Explanation:

The answer would be B!

The overall equation for cell respiration is oxygen glucose ----> carbon dioxide water energy. if you flip this equation in reverse, what process does that equation most resemble?

Answers

he overall equation for cell respiration is oxygen + glucose → carbon dioxide + water + energy. If we flip this equation in reverse, it will look like the equation for photosynthesis.Cellular respiration and photosynthesis are two interconnected metabolic processes.

Both cellular respiration and photosynthesis are vital to life on Earth. During photosynthesis, plants transform light energy into chemical energy stored in organic compounds. Cellular respiration is a metabolic process that transforms organic compounds into energy (ATP). Photosynthesis converts carbon dioxide and water into glucose and oxygen. In contrast, cell respiration oxidizes glucose to yield ATP while producing carbon dioxide and water.A common misunderstanding is that photosynthesis is the opposite of cellular respiration.

The reactions for photosynthesis and cellular respiration are not exact opposites. Photosynthesis involves the absorption of light by pigments, whereas cellular respiration does not. Cellular respiration requires oxygen and gives off carbon dioxide, whereas photosynthesis requires carbon dioxide and gives off oxygen.

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An independent state made up of a city and the land around it? (I don’t get it we just started this)

Answers

It is a city that has its surrounding territory forms an independent state.

A city-state is an independent city — and sometimes its surrounding land — which has its own government, completely separate from nearby countries.

Example of this is Monaco.

How does the muscular system interact with the digestive system?
A.
Food is moved through the small intestine.

B.
Oxygen is moved into and out of the lungs.

C.
Saliva production increases at the sight or smell of food.

D.
Nutrients are absorbed and transported to other body parts.

Answers

Answer:

food is moved through the small intestine

Describe how the skin cells, neurons, muscle cells, and blood cells you have observed relate to the functions of skin, nerve, muscle, and blood tissue.

Answers

Answer:

The form makes the function, this saying is named by many scientists, and they were the ones who made a relationship with the physiology of the organ or tissue and the shape of the cells that make it up.

Explanation:

In the case of neurons, they have extensions to spread and transmit signals, epithelial cells form many layers and have the ability to shed, that is why their main function is the body's innate protective barrier.

Red blood cells are double-headed and enucleated, to be made up of proteins that conform to oxygenation and thus diffuse oxygen well throughout the body.

And finally the muscle cells, which are tapered, with internal sarcomeros, similar to an elastic, to be able to carry out effective contractions starting from a certain length.

what compares nucleic acids and carbohydrates

Answers

Answer:

D. They both contain carbon, but only nucleic acids contain phosphorous.

Explanation:

when excess macromolecules are converted to triglycerides and stored in fat cells.

Answers

Excess macromolecules are stored in the form of triglycerides in fat cells.

When we consume more calories than our body needs, the excess macromolecules are converted into triglycerides by a process called lipogenesis. These triglycerides are then stored in adipose tissue as a form of energy reserve. When the body needs energy, it can break down these triglycerides through a process called lipolysis. However, if we continue to consume more calories than we burn, these triglyceride stores can become too large, leading to obesity and other health issues. Therefore, maintaining a healthy diet and regular exercise routine is essential for preventing the accumulation of excess triglycerides in the body.

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On orders from the cerebral cortex (via the _________ and _______ gland), the outer part of the adrenal glands secrete glucocorticoid stress hormones such as cortisol.

Answers

Answer:

I suppose it's via the hypothalamus and anterior pituitary gland

explain how sexual reproduction increases genetic diversion in many species of plants and animal, making sure to include the importance and function of gamites

Answers

Answer:

Sexual reproduction increases genetic diversion because there are two parents, unlike in asexual reproduction where there is only one.

Explanation:

During sexual reproduction, gamites come from both parents and mix together to create genes and traits for the offspring. Gamites are formed through meiosis (reduction division), in which a germ cell undergoes two fissions, resulting in the production of four gametes.

what is the function of amniotic fluid?group of answer choicesprotects the embryo against being jostled by movements.stores nutrients for the fetus.provides nutrients to the fetus from the maternal part of the placenta.absorbs wastes the fetus excretes.

Answers

The function of amniotic fluid is protects the embryo against being jostled by movements.

The function of amniotic fluid includes the following:

1. Protects the embryo against being jostled by movements: The amniotic fluid protects the developing fetus from jolts and injuries due to its fluidic environment. As a result, if the mother experiences a fall or sudden movement, the fetus will be shielded by the amniotic fluid, which will absorb the shock. The amniotic fluid maintains a stable temperature, ensuring that the fetus is comfortable and healthy.

2. Absorbs wastes the fetus excretes: The amniotic fluid serves as a waste disposal system for the developing fetus, absorbing and carrying away all of the waste products that the fetus generates. The fetus urinates the fluid into the amniotic sac, and the amniotic fluid carries it away.Apart from these, the amniotic fluid plays a crucial role in fetal development by protecting the developing fetus from physical injury and infection. It also keeps the temperature stable and provides an excellent environment for the fetus to grow and develop healthily.

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1. Which systems (2) are primarily responsible for movement of the body

Answers

Answer:

The correct answer is -muscular system and skeletal system.

Explanation:

The skeletal system and the muscular system work together to move the body or body parts. Different types of tissues form the muscular system that and their action on bones controls the movement of the body in vertebrates.

Tendons are tough muscles that attach with the bones and joints allow the movement by contraction of muscle that helps in moving bones from joint. Muscle normally acts by contracting and relaxing in pairs.

If the sugar concentration in a cell is 4% and the concentration in a cell’s environment is 2%, how can the cell obtain more sugar?

Answers

For a cell to obtain more sugar the cell must use active transport to pump the sugar inside it .

Active transport is the movement of molecules across a cell membrane from a region of lower concentration to a region of higher concentration which occur against the concentration gradient . So , it requires cellular energy to achieve this action.

Main function of active transport is involves the movement of molecules from a region of lower concentration to a region of higher concentration with help of energy in form of ATP. examples of active transport include the absorption of glucose in the intestine of the human body and the uptake of minerals or ions into the root hair cells of plants.

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3
ANNOTATE Write the inputs and outputs of cellular respiration on this diagram.

3ANNOTATE Write the inputs and outputs of cellular respiration on this diagram.

Answers

Answer:

Inputs---- glucose and oxygen.

Outputs----- ATP, carbondioxide and water.

Explanation:

The inputs of cellular respiration are the glucose and oxygen whereas the outputs of cellular respiration are energy in the form of ATP, carbondioxide gas and water. cellular respiration is a process in which glucose is broken down in the presence of oxygen gas for the production of energy in the form of ATP molecules. Carbondioxide gas and water which are the waste materials also produced in the end of cellular respiration process. Cellular respiration is the reverse of photosynthesis.

what did Chargaff find that the number of Gs in the DNA equaled to?

Answers

Chargaff discovered that in DNA, the number of guanine (G) bases is equal to the number of cytosine (C) bases. This is known as Chargaff's rule and it is essential for the proper pairing of DNA strands during replication.

Chargaff's rules state that in the DNA of any species and any organism, the amount of guanine should be equal to the amount of cytosine and the amount of adenine should be equal to the amount of thymine. Further a 1:1 stoichiometric ratio of purine and pyrimidine bases (i.e., A+G=T+C) should exist. This pattern is found in both strands of the DNA.

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The base bnce is 1 Animals in an experiment are to be kept under a strict diet. Each animal should receive 25 grams of protein and 5 grams of fat. The laboratory technician is able to purchase two food mixes: Mix A has 10% protein and 6% fat; mix B has 20% protein and 2% fat. How many grams of each mix should be used to obtain the right diet for one animal?

Answers

The right diet for one animal, 180 grams of Mix A and 35 grams of Mix B should be used.

To calculate the amount of each food mix needed to achieve the desired protein and fat content in the animal's diet, we can set up a system of equations based on the given information.

Let's assume x grams of Mix A and y grams of Mix B are used.

The protein content from Mix A can be calculated as 10% of x grams, which is 0.1x grams. Similarly, the protein content from Mix B is 20% of y grams, or 0.2y grams.

The fat content from Mix A is 6% of x grams, or 0.06x grams, and the fat content from Mix B is 2% of y grams, or 0.02y grams.

We need the total protein to be 25 grams and the total fat to be 5 grams.

From the given information, we can set up the following equations:

Protein equation: 0.1x + 0.2y = 25 (equation 1)

Fat equation: 0.06x + 0.02y = 5 (equation 2)

To solve this system of equations, we can use various methods, such as substitution or elimination. Here, we'll use the substitution method.

First, isolate one variable in equation 1. Let's solve for x:

0.1x = 25 - 0.2y

x = (25 - 0.2y) / 0.1

Substitute the value of x in equation 2:

0.06((25 - 0.2y) / 0.1) + 0.02y = 5

Simplify and solve for y:

1.5 - 0.12y + 0.02y = 5

-0.1y = 3.5

y = -3.5 / -0.1

y = 35

Now substitute the value of y back into equation 1:

0.1x + 0.2(35) = 25

0.1x + 7 = 25

0.1x = 18

x = 180

Therefore, to obtain the right diet for one animal, 180 grams of Mix A and 35 grams of Mix B should be used.

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which technology has been commonly utilized by scientists to manipulate heritable information by producing large amounts of identical recombinant dna molecules? gel electrophoresis gene cloning artificial selection restrictive enzyme analysis

Answers

The technology that has been commonly utilized by scientists to manipulate heritable information by producing large amounts of identical recombinant DNA molecules is gene cloning.

Gene cloning is the process by which we insert a section of DNA into a cell and then let the cell undergo mitosis, creating copies of that genetic information. Gene cloning is different from organism cloning, which uses an organism's DNA to produce a genetically identical organism because it only produces cells, not an entire organism.

Cloning of any DNA fragment involves four steps in the traditional restriction enzyme digestion and ligation cloning protocols: isolation of the desired DNA (or target DNA ), ligation, transfection, or transformation.

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What causes a neutral mutation?

Answers

Answer:

Like when something changes but doesnt affect us in a good or bad way. like when the color of human hair changes, it wont change us genetically.

Explanation:


A collision between which two types of crust will most likely produce a mountain range?

Answers

Answer:

Be more specific

Explanation:

Answer:

A convergent boundary

Explanation:

Convergent boundaries are pieces of crust that collide against each other. They push upwards, and that land that is pushed upwards is a mountain. Mount Everest is a good example. It was made in two tectonic plates, and since these plates are still pushing upwards to this day, this mountain grows around 40 cm each year. I hope this helps!

seminal vesicles. secretes 60% of semen volume; alkaline pH; prostaglandins. testes. organ that produces sperm. membranous urethra.

Answers

60% of semen is secreted by seminal vesicles. Membranous urethra is component of male reproductive system and testes create sperm.

60% of the semen volume, which has an alkaline pH and contains prostaglandins, is secreted by the seminal vesicles. The membranous urethra is a component of the male reproductive system, whereas the testes are the organs that create sperm.

The seminal vesicles are a pair of glands in males that are situated close to the base of the bladder. By secreting around 60% of the volume of semen, they contribute to its composition. Fructose, proteins, enzymes, and prostaglandins are among the chemicals found in the seminal fluid that the seminal vesicles create. The urethra and female reproductive tract's acidic environment is partially offset by the seminal fluid's alkaline pH, creating a favourable environment for sperm survival and motility. The seminal fluid contains prostaglandins.


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How does this model support the conclusion that cells need to have a high amount of surface area relative to their total volume?

Answers

Answer:

Due to the increase in surface area, the nutrients move efficiently inside the cell.

Explanation:

The cells need to have a high amount of surface area as compared to their total volume because, with the increase of surface area, the nutrients move efficiently inside the cell. A large surface area to volume ratio indicates that the cell can be efficient in moving nutrients and waste materials around using the process of diffusion but when the volume increases instead of surface area, the efficiency of the cell decreases, and cells won't be able to do its job efficiently.

The space inside a table cell between the text and the cell borders.

Answers

Answer:

Cell margin. The space inside a table cell between the text and the cell borders-top, bottom, left, and right. Column Width.

Imagine that one part of the Great Pacific Garbage Patch consists completely of plastic
bottle caps. The bottle caps cover a region that is roughly 5 cm deep. 100 cm in the
north-south direction, and 200 cm in the east-west direction. What is the volume filled
by these bottle caps, in cmº?

Answers

Answer:

100,000 cm is what it covers

in general, a muscle that crosses on the anterior side of a joint produces ________.

Answers

Answer: Flexion

Explanation:A muscle that crosses on the anterior side of a joint produces flexion. A muscle that crosses on the posterior side of a joint produces extension.

Answer: Flexion

Explanation:

3. Muscles have 3 important functions: _______of body parts, maintenance of________
and production of body______.

Answers

Answer:

Movement of body parts.

Maintenance of posture.

Production of body heat.

Explanation:

Your welcome! :)

Based on your observations, how many cells are in anaphase?

Answers

Answer:

D.19 There are 19 in the anaphase

Explanation:

A liquid that is being heated at Lakesha’s lab table catches fire. Which action should Lakesha take to best ensure the safety of her and her classmates?

Call 911 on a mobile phone.

Leave the lab table to find the fire alarm.

Look for the fire extinguisher to put out the fire.

Alert everyone in the lab while stepping away from the fire

Answers

Look for the fire extinguisher to put out the fire

Which of these options represents the correct flow of events during meiosis II?
A.
Sister chromatids pulled to opposite poles, nucleoli form, cytokinesis
B.
Sister chromatids pulled to opposite poles, nuclear envelope disappears, cytokinesis
C.
Cytokinesis, sister chromatids pulled to opposite poles, nucleoli disappear

Answers

Answer:

B

Explanation:

that seems to be the best one

A goal of rapid-transit systems is to decrease air pollution. True False

Answers

True

Have a good day

What type of molecule did you make?

AUG GGU UGU UAA

Answers

AUG GGU UGU UAA is an amino acid sequence, which further forms proteins.

What are amino acids?

Proteins are made up of substances called amino acids. Proteins and amino acids are the components of life. Amino acids are the byproducts of the digestion or breakdown of proteins.

Amino acids are used by the human body to create proteins that aid in dissecting food.

There are mainly 20 types of amino acids. 10 are essential amino acids, while 10 are non-essential amino acids. Our body synthesizes non-essential amino acids, while we have to include essential amino acids in our diet.

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Exercise 1 Underline the correct word in each sentence.People cant hardly walk when sidewalks are covered with ice. The ______________ did not agree with this and instead decided they no longerwould be a part of the United States at all--they would ______________. In1861, just when President Lincoln was taking office, seven southern states left theUnion and started their own new country: the __________________________of America. Before long, four more states joined them in leaving the Union. Answer correct with no website the correct answer Object's velocity changeHow you made this change1. start moving2. speed up3. slow down4. stop moving5. move in opposite directionCan somebody help me fill this chart out pls Which function will produce the same graph as the function shown below?v=(r+1) (r+3)OAVt3 =x(r- 1)O B.-3 = (r- )Oc.v=xt4r- 3OD.V=r+4r+3 Endotherm metabolic rates are steady (unchanging), regardless of changing ambient temperature, as long as the body temperature is normal.TrueFalseFalse but only for mammals, true for snakes. Why did the Bill of Rights have less influence over American life in the 19th century?The Supreme Court ruled that it only applied to the federal government.American culture was biased against individual freedom at the time.Presidents frequently kept the Supreme Court busy with other issues.Leaders couldn't decide whether it should also apply to new states. Read the step for determining the amino acids based on codons .Use that figure to answer the questions that follow(mark brainliest if you explain how to read the chart wheel thing) as he drove out of the driveway, he saw his wife waving but did not realize that she wanted him to stop to avoid running over the tricycle. he thought she was just waving good-bye. the problem in this nonverbal episode is that we which complication would the nurse associate with the long term use of indwelling catheters select all the apply What is the theme of the central idea of the story? (10+10+10 pts.) Determine whether each of the following is linearly dependent / independent. (i) {e22, 1, em } (ii) {tan? (x), sec+ (x), 1} 1 2 0 0 21 (iii) 1 1 {[; 16_3} C _1] [21] 2 - 2. 5 Justify y How many address lines and input output data line in 2K x 16? Show your solution: When humid air rises, clouds often form. Explain why the rising air causes cloud formation. Biozone provides its employees career counseling, skills workshops, and networking sessions. This work-life initiative used by Biozone falls under the category of _______ notch is a receptor protein displayed on the surface of certain cells in developing fruit fly embryos. notchs ligand is a membrane-bound protein called delta that is displayed on the surface of adjacent cells. when notch is activated by its ligand, the intracellular tail of the notch protein becomes separated from the rest of the protein. this allows the intracellular tail to move to the cells nucleus and alter the expression of specific genes. an investment of $25 at an annual rate of 10% will return a higher value in five years than $50 invested at an annual rate of 5% in the same time. If P and Q are complementary angles and Q is 66, what is the measure of P? An African elephant weighs 2 tons. How many pounds are in a herd with 26 elephants? _____ mainly affect alcoholics and those with cirrhosis of the liver.A. intussusceptionB. hemorrhoidsC. adhesionsD. esophageal varices Can someone help me please