) you are decellularizing a porcine artery for use as a small diameter vascular graft. what in vitro assay(s) could you use to determine whether the resulting decellularized xenogeneic tissue would be rejected by a human host

Answers

Answer 1

Decellularized porcine saphenous artery for small diameter tissue engineered conduit grafts.

Overview:

Decellularized xenografts have been identified as potential scaffolds for small-diameter vascular grafts. The purpose of this study was to biomechanically transform the porcine decellularized saphenous artery (DPSA) by a modified decellularization process using a Triton X-100/NHOH solution and serum-containing medium.

The goal was to develop and explore functional, biocompatible acellular conduits. Histological and biochemical analyzes showed a high degree of cell clearance and extracellular matrix preservation. Burst pressure tests showed that DPSA can withstand pressures of 1854 ± 164 mm Hg.

In vitro cell adhesion and biocompatibility evaluations showed that porcine pulmonary artery endothelial cells can adhere and grow on DPSA in static and rotary cultures. After implantation into the rabbit carotid artery in vivo, DPSA showed a patency rate of 60% at 1 month and 50% at 3 months.

No aneurysm and intimal hyperplasia were observed with any of his DPSA. All patented grafts showed regeneration of vascular elements and thrombotic occlusion was found to be the major cause of graft failure.

This is probably due to residual xenoantigens. In summary, this study demonstrated the development and evaluation of a decellularization process that could be used as a small-diameter graft.

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Related Questions

Define plumule, radicle, coleoptile, coleorhiza, hypocotyl, after-ripening, stratification, and vivipary.

Answers

Plumule: Embryonic shoot; Radicle: Embryonic root; Coleoptile: Sheath covering shoot; Coleorhiza: Sheath covering root; Hypocotyl: Connection between root and shoot. After-ripening: Maturation of dormant seeds. Stratification: Controlled seed exposure to break dormancy. Vivipary: Seeds germinate while attached.

1. Plumule: The embryonic shoot of a plant found within a seed, which eventually develops into the stem and leaves.

2. Radicle: The embryonic root of a plant found within a seed, which grows downward to anchor the plant and absorb water and nutrients from the soil.

3. Coleoptile: A protective sheath that covers the emerging shoot (plumule) in certain monocot plants, aiding in the penetration of the soil during germination.

4. Coleorhiza: A protective sheath that surrounds the radicle in certain monocot plants, facilitating the penetration of the soil during germination.

5. Hypocotyl: The region of a plant embryo situated between the radicle and the cotyledons, which serves as the connection between the root and the shoot.

6. After-ripening: The process in which dormant seeds undergo a period of maturation or aging, leading to changes in their physiological state that promote successful germination and growth.

7. Stratification: The controlled exposure of seeds to specific environmental conditions, such as cold and moist conditions, to simulate natural winter conditions and break seed dormancy, enhancing germination.

8. Vivipary: A reproductive adaptation in certain plants where seeds germinate while still attached to the parent plant, allowing them to establish themselves before being dispersed.

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What are the three types of interactions between organisms in an ecosystem? a. competition, mutualism, and predation b. territoriality, predation, and symbiosis c. competition, predation, and symbiosis d. commensalism, parasitism, and mutualism please select the best answer from the choices provided a b c d

Answers

Competition, predation, and symbiosis are the three types of interactions that occur between organisms in an ecosystem. Therefore option c is the correct choice.

Competition occurs when organisms are competing for the same resources. It can be between organisms of the same species (intraspecific competition) or different species (interspecific competition). The act of eating all or a portion of the body of another organism, the prey, is known as predation. A relationship or interaction between two separate organisms that live in the same habitat is referred to as symbiosis. Three different forms of behavior, known as mutualism, commensalism, and parasitism, are seen in symbiotic interactions.

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striking the funny bone is actually stimulation (of injury) to which plexus

Answers

Striking the funny bone is actually stimulation (of injury) to the ulnar nerve in the cubital fossa, which is associated with the brachial plexus.

The term "funny bone" refers to the ulnar nerve, which runs along the inside of the elbow. When the elbow is struck or compressed, such as hitting it against a hard surface, it can cause a tingling or electric shock-like sensation in the forearm and hand. This sensation is commonly known as hitting the funny bone, although it is not actually a bone.

The ulnar nerve is a major nerve that originates from the brachial plexus, a network of nerves that originates from the spinal cord in the neck region. The brachial plexus consists of nerves that innervate the upper limb, including the arm, forearm, and hand.

Therefore, striking the funny bone is actually stimulation or injury to the ulnar nerve, which is associated with the brachial plexus. This nerve irritation can cause temporary discomfort and a tingling sensation in the forearm and hand.

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20 POINTS + A BRAINLIEST!!!

20 POINTS + A BRAINLIEST!!!

Answers

Answer:

its 10 points but it's fine

----------------------

The answer is C

Explanation:

Answer:

I think the answer is C, so sorry if I got it wrong.

n
Ascientist performs an investigation and discovers that increased temperature decreases the number of offspring that an
organism produces. Then, the scientist asks a new question about the impact of climate change on the species because
O the scientist's first question was not a scientific question.
the scientist modified the hypothesis for the investigation.
the results of the investigation led to new scientific questions,
questions about the impact of temperature reflect an opinion.
Mark and retum
Save and Exit
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Submit
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Answers

Answer:

the results of the investigation led to new scientific questions

Explanation:

The basis of every scientific question asked is an observation made. Scientific questions are questions that emanate from an observed problem and leads to the formation of a testable explanation (hypothesis). In this question, an investigation was made by a scientist and it was discovered that increased temperature decreases the number of offspring that an organism produces.

However, the scientist asks a new question about the impact of climate change on the species. This is because an observation has been made again, from the previously concluded investigation. Hence, after completing the investigation about the effect of temperature on the number of offsprings an organism can produce, an observation was made, ultimately leading to a new scientific question.

Answer:

c

Explanation:

please please please help me on me I need it ASAP ​

please please please help me on me I need it ASAP

Answers

The answer is
1. C
2. B

Which is an example of a scientific theory?
A. On average, larger mammals sleep longer than smaller mammals.
B. If a plant receives pure water, then it will grow more than one that
receives salt water.
C. Organisms evolve over time due to changes in heritable physical
characteristics.
D. If a person takes at least one vitamin per day, then it will reduce a
person's level of tiredness.
HELP PLEASE!

Answers

Answer: Organisms evolve over time due to changes in heritable physical characteristics.

Explanation:

There are a few things to look for when distinguishing between a nonscientific theory and a valid scientific theory. One thing to consider is if there are any published literature, experimental studies, and data available to support the theory. Most scientific theories have data and information tied to them. Another factor to examine is who has developed the theory. A scientific theory will always be supported by evidence with large quantities of data and testing. An example of a scientific theory is the theory of evolution, which observes changes in heritable physical traits over time in an organism. A hypothesis might be that all organisms evolve at the same rate.

Organisms evolve over time due to changes in heritable physical characteristics is an example of a scientific theory known as evolution. Thus, option C is correct.

What is evolution?

Evolution is the scientific theory that explains that "a change in the characteristics of biological populations over next generations.

According to scientists RNA is the first existing molecule on earth that replicate by itself and the process of evolution lead to advanced forms of life.

These characteristics are easily trasfered from one generation to other generation.

Therefore, Organisms evolve over time due to changes in heritable physical characteristics is an example of a scientific theory known as evolution. Thus, option C is correct.

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how do phagocytes recognize foreign cells or bacteria?

Answers

Foreign particles can also be identified by phagocytes by the binding of soluble molecules to the particles, marking them for ingestion.

Once on the surface of the target particle, these chemicals, known as opsonins, are detected by phagocyte membrane receptors. Many receptors on the plasma membrane of phagocytic cells identify these particles and activate the phagocytosis process. A single phagocyte can express many receptor types, which work together to recognize and consume the particle.

Invaders are recognised by their antigens, which are proteins on the surface of invading cells. Every cell or substance has its own antigens, and a person's cells include "self-antigens" that are particular to that person.

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what is assembly drawing
olease provide the example of assembly drawing

Answers

An assembly drawing is a technical drawing that represents the arrangement and relationship of various parts and components in a mechanical or engineering assembly.

An assembly drawing provides a visual representation of how different parts fit together to create a complete product or system. Assembly drawings are typically used in manufacturing and engineering processes to guide the assembly and production of complex structures or machines.

In this example, the assembly drawing shows a "Widget Assembly." It consists of several components represented by rectangular boxes, which are labeled as "Component." The lines and arrows indicate the relationships and connections between the components, depicting how they fit together to form the complete assembly.

Assembly drawings often include additional information such as part numbers, dimensions, and annotations to provide further details for assembly and manufacturing processes.

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what is assembly drawingolease provide the example of assembly drawing

In a
circuit, the current is the same everywhere.​

Answers

In a  Series circuit, the current is the same everywhere.​

the attachment point of two sister chromatids is called the

Answers

The attachment point of two sister chromatids is called the centromere.

A centromere is a specialized structure located in the center of a chromosome that connects the two sister chromatids, which are produced during replication. A centromere, a specialized DNA sequence, is responsible for this crucial attachment.

Furthermore, this region plays a critical role in the assembly of kinetochores, which are responsible for the correct attachment and segregation of chromosomes during mitosis.The point at which two chromatids join and form a 'X' shape is the centromere. The centromere is a crucial part of the chromosome since it provides a location for the spindle fibers to attach. The spindle fibers then separate the two chromatids during cell division.

As a result, the centromere is critical for maintaining the chromosome's integrity and promoting the equal segregation of genetic material during cell division.

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What skin condition requires you to treat the whole family?

Answers

There are several skin conditions that may require treating the entire family, including contagious conditions like scabies, head lice, and ringworm.

Treating the whole family helps prevent the spread and recurrence of these conditions. Certain skin conditions have the potential to spread easily within a household, making it necessary to treat the entire family. One example is scabies, a highly contagious skin infestation caused by microscopic mites. Scabies can be transmitted through close physical contact and shared bedding or clothing. Treating all family members simultaneously helps ensure that the mites are eradicated and prevents reinfestation.

Another common condition that requires treating the whole family is head lice. These tiny insects infest the hair and scalp, causing itchiness and discomfort. Head lice can easily spread from one person to another, especially in close quarters such as households. Treating all family members at the same time helps eliminate lice and their eggs, reducing the chance of reinfestation.

Ringworm is another skin condition that can affect multiple family members. It is a fungal infection that causes circular, red, and itchy patches on the skin. Ringworm can be spread through direct contact with an infected person or by sharing personal items like towels, combs, or clothing. Treating all family members helps eliminate the fungus and prevents its spread within the household.

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does cell division in both bacterial and eukaryotic cells involves the replication and segregation of information and the division of contents within the cell?

Answers

Yes, cell division in both bacterial and eukaryotic cells involves the replication and segregation of genetic information and the division of cellular contents within the cell.

However, the mechanisms of cell division differ significantly between bacterial and eukaryotic cells.

Bacterial cells divide by binary fission, which is a simple process that involves DNA replication, elongation of the cell, and finally, the division of the cytoplasm and the cell membrane to form two identical daughter cells.

In contrast, eukaryotic cells undergo a more complex process of cell division called mitosis, which includes several distinct stages such as prophase, metaphase, anaphase, and telophase, leading to the separation of chromosomes and the division of the cytoplasm to form two identical daughter cells.

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What is the main difference between passive
transport and active transport of materials across a
cell membrane?

Answers

Answer: The main difference is whether the transportation requires energy or not.

Explanation: Active transport most of the time requires energy, passive transport does not.

what is the significance of black eyed peas and cabbage for new years

Answers

Black eyed peas are for good luck and Cabbage is for money

Fill in the blank lines in the diagram above to label the two types of connective tissue
shown, then describe the function of each below.

Fill in the blank lines in the diagram above to label the two types of connective tissueshown, then describe

Answers

Answer:

ligaments connect one bone to each other while tendon connect muscle to bone

Answer:

The first line that connects bone to the biceps, which is a muscle, is a tendon since the tendon connects bone to muscle while the other line down there connects bone to bone which means it is a ligament.

Explanation:

Consider an individual with red-green colour deficiency.What is the minimum possible number of the child's GRANDPARENTS who were also red-green colour deficient?*note* Please enter numbers only - (0-4)

Answers

As the character is recesive and humans are diploid, the idividual must have both alleles with the information for red-green colour deficiency if she is a woman, or the only allele with that information if he is a man; this is because the gene for colour deficiency is located on the sexual chromosome X.

Therefore, if both grandmothers have the allele for colour deficiency but they have a heterozygous genotype, neither of the grandparets would have colour blindness, even though, they could give their offspring the red-green colour deficiency allele.

To sum up, 0 grandparents can be red-green colour deficient (but at least one must have the corresponding allele) and have a grandson/daughter with red-green colour deficiency.

Blood testing for presence of Chlamydia immunoglobulins. Report code _____.
a. 86631
b. 86632
c. 86705
d. 86790

Answers

The report code for blood testing for the presence of Chlamydia immunoglobulins is option A, which is 86631. This code is used to identify the procedure of detecting antibodies produced by the immune system in response to Chlamydia infection.

The test involves drawing a blood sample from the patient and analyzing it for the presence of Chlamydia-specific immunoglobulins, which indicate a past or current infection. This test is useful in diagnosing Chlamydia in patients who do not present with typical symptoms. It is also important in monitoring treatment progress and determining the need for further testing or treatment. It is important to use the correct report code to ensure proper billing and reimbursement for this diagnostic test.
You're asking about the correct report code for blood testing for the presence of Chlamydia immunoglobulins. The correct report code for this test is:

a. 86631

To summarize, when conducting a blood test for Chlamydia immunoglobulins, you should use report code 86631.

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What occurs in the light independent reactions?

Answers

Answer:

Light energy is harnessed in Photosystems I and II, both of which are present in the thylakoid membranes of chloroplasts. In light-independent reactions (the Calvin cycle), carbohydrate molecules are assembled from carbon dioxide using the chemical energy harvested during the light-dependent reactions.

Explanation:

what is the scientific name of wise​

Answers

Judicious, prudent, and sage

Does anyone know the answer?

Does anyone know the answer?

Answers

Answer:

C. Sunlight penetrates to greater depths in land than water

Explanation:

Water is a poor conductor of heat which is why it takes much longer for water to conduct heat than it does land.

I hope this helped :)

hi i really need help on this

hi i really need help on this

Answers

Answer: Help is Here!

Explanation:

Once every 24 hours Earth fully with us on it. When we are on the side of Earth that is facing the Sun, we have daylight from the sun. As Earth continues its spin, we are moved away from our Sun, and we have night time because there is no more light from the sun (on our side).

i need help for these 2 questions , the second question options are

A. mutualism
B. predation
C. parasitism
D. competition ​

i need help for these 2 questions , the second question options are A. mutualism B. predation C. parasitism

Answers

Answer:

For No. 3 question

Mutualism (A)

Parasitism (C)

What factor(s) can affect diffusion speed?

None of the answer choices affect diffusion speed.

size of the diffused materials

shape of the diffused materials

size of the diffused materials and shape of the diffused materials

Answers

Answer:

C. Size of the diffused materials and shape of the diffused materials

design a controlled experiment to determine whether earthworms change the forest ecosystem. identify the environmental factor you will measure, state the specific hypothesis you will test, and design a procedure for collecting data accurately.

Answers

Hypothesis for a controlled experiment to determine whether earthworms change the forest ecosystem, The soil's PH will drastically change after the worms consume the leaf litter in a closed container filled with soil.

A number of plots of deciduous forest land would be the subject of an investigation in a controlled experiment to ascertain the effects of the new invasive worm. To make it easier to create artificial ecosystems in a lab, each plot should have boundaries that are limited. There ought to be three main groups: one without any worms, one with only native worms, and one with worms from both native and exotic species. similar circumstances ought to be applied to every individual plot: the same number of trees, the same amount of initial leaf litter, and the same amount of precipitation. The mass of the leaf litter should be taken at the conclusion of the experiment and compared to the initial mass. The plot with the exotic worms is anticipated to have the least amount of leaf litter compared to the other plots. The premise is: The amount of leaf litter that remains at the end of a season in a plot with only the native worm species will be significantly less if an exotic worm species is introduced into a deciduous forest ecosystem. To guarantee accurate results, multiple trials should be carried out.

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4
12.5 points
Using coal for energy is damaging to the environment because
Sev3b
coal mining requires digging deep into Earth's crust which causes fracking
leaks during mining can cause spills that pollute the ocean
O
many coal piants have had accidents that released radiation into the air
burning of coal produces air pollution and the greenhouse gas CO2

Answers

Using coal for energy is damaging to the environment because D. burning of coal produces air pollution and the greenhouse gas CO2

How is coal for energy harmful?

The most significant, long-term global impact of coal is climate change. Chemically, coal is mostly made up of carbon, which when burned produces carbon dioxide, a gas that traps heat.

The blanket-like effect of carbon dioxide, which is released into the atmosphere, causes the earth to warm more than usual, hence, this is the danger to the environment.

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1) The transplantation of a normal gene into a cell in place of missing or defective ones in order to correct a genetic disorder.
A) Genetic Modification
B) Genetic Engineering
C) Gene Therapy
D) Gene Splicing

2) Aspects of the body that help organisms survive and reproduce
A) Structural Adaptation
B) Mimicry
C) Behavioral Adaptation
D) Camouflage

Answers

Answer:

1. B

2. A

I think

Explanation:

Ask me anything I’ll answer if I can

Answers

Answer:

can you answer my most recent question please

Explanation:

In an attempt to identify ecologically friendly methods for controlling mosquito​ populations, researchers conducted field experiments in a certain country where aquatic nymphs of the dragonfly brachytron pretense were used against the larvae of mosquitoes. the researchers concluded that brachytron pretense can be used effectively as a​ strong, ecologically friendly control of mosquitoes and​ mosquito-borne diseases. complete parts​ (a) through​ (i).

Answers

Answer:

Mosquito fish.

Explanation:

Another ecologically friendly methods such as mosquito fish is also used for controlling mosquito​ populations. Mosquito lays egg in the ponds so these mosquito fish feeds on the larvae of mosquito and remove or reduce the population of larvae so very less number of larvae change into adult so this method is also a very good method for controlling of mosquito and no bad effect on the environment.

Dose mitochondria transfer things to the cell

Answers

Answer:

Yes, mitochondria transfers energy to the cell.

Explanation:

A cell can be defined as the fundamental or basic functional, structural and smallest unit of life for all living organisms. Some living organisms are unicellular while others are multicellular in nature.

A unicellular organism refers to a living organism that possess a single-cell while a multicellular organism has many (multiple) cells.

Muscle cells use more energy than most other cells in the body. Because of this, muscle cells must have more mitochondria.

Basically, mitochondria is one of the cell organelles found in all living organisms and it is known as the powerhouse. Therefore, mitochondria provides all the energy required in the cell by transforming energy forms through series of chemical reactions; breaking down of glucose into Adenosine Triphosphate (ATP) used for providing energy for cellular activities in the body of living organisms.

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Which function of a research introduction is often underdeveloped in research proposals?. three weeks after a river rafting trip, three family members experienced symptoms of coughing, fever, and chest pain. during the rafting trip, the family had consumed crayfish that they caught along the river banks. an examination of the patients' sputum revealed helminth eggs, and serum samples were positive for antibodies to paragonimus. all of the family members recovered following treatment with praziquantel. in the paragonimus life cycle, a) the crayfish are the definitive host and humans are the intermediate host. b) humans are the definitive host and crayfish are the intermediate host. c) both humans and crayfish are intermediate hosts. d) both humans and crayfish are definitive hosts. e) the source of the infection was the river water. An object is dropped from rest from a 70.6 m tower. Air resistance is negligible. After 0.32 seconds, what is magnitude and direction of its acceleration? A change that is useful for the environment and living things is called When deciding whether to fly or take the train on a trip, the cost of putting your pet in a boarding facility while you are away is a(n) Blank______ cost. Multiple choice question. sunk relevant irrelevant Keyser Mining is considering a project that will require the purchase of $980,000 in new equipment. The equipment will be depreciated straight-line to a zero book value over the 7-year life of the project. The equipment can be scraped at the end of the project for 5 percent of its original cost. Annual sales from this project are estimated at $420,000. Net working capital equal to 20 percent of sales will be required to support the project. All of the net working capital will be recouped. The required return is 16 percent and the tax rate is 35 percent. What is the amount of the after-tax salvage value of the equipment? A. $17,150B. $31,850C. $118,800D. $237,600E. $343,000 For the following scenarios, compute the maximum total deductible contribution to the the traditional IRA contributions for 2015. Juan, age 41, earns a salary of $28,000 and is not an active participant in any other qualified plan. His wife, Agnes, has no earned income. Juan wishes to contribute as much as possible to his own IRA. please help me, quick and right answers only algebra :) I have test pleas helpWhich of the following statements does not form part of the "cell theory"? a) All living organisms are made of cells. b) The cell is the basic functional unit of all living organisms. c) All plants are made of cells, but only certain types of animals are. d) All cells come from pre-existing cells. What are the local, state, and national governments that have control over the community in which you live?I'll mark you brainliest!!!!! The blueprint for a new house includes a triangular shaped room in the attic. The triangular room appears on the blueprint as shown. If the blueprint was made using a scale factor of12centimeter = 1 meter, what is the actual perimeter of the triangular room? In the figure below, the segments are tangent to the circle and CD = y - 9 and ED = 2/3 yFind CD. A narrow waterway between islands is calleda straita sounda inletan isle b - 7 = -1One set equation with integers In circle Q, m=120RQS=120 and the area of shaded sector = 33. Find the length of RTS. Express your answer as a fraction times Refer to case study titled Patanjali Ayurveda An Unconventional Business Machine and answer questionsQ2. What is Patanjalis competitive advantage? How would you go about executing a strategy for a company for which Business is a by-product? Discuss the 10-basic task of the strategy execution process in this regard? Calculate the mean of 59,91,87,64,84 What did the artist foresee as an outcome of the policies of the United States?A)The League of Nations would be a powerful organization without theUnited States.B)Nations the United States fought in World War I would takeleadership roles in the League of Nations.C)The League of Nations would struggle to function without theinvolvement of the United States.D)Other countries would break diplomatic ties with the United States ifit did not join the League of Nations. The volume of a gas cylinder contains 7.40l of nitrogen-gas under a pressure of 2.972 atm at 51.8 c. calculate the number of molecules of nitrogen in the tank how dose the king begin the letter? what is the impact of king's choices?